Yuan Xuzhao, Luan Yanfang, Liu Dong, Wang Jian, Peng Jianxiang, Zhao Jinlei, Li Lupeng, Su Jingjing, Xiao Yang, Li Yuanjie, Ma Xin, Zhu Xiaoyang, Tan Lubin, Liu Fengxia, Sun Hongying, Gu Ping, Xu Ran, Zhang Peijiang, Zhu Zuofeng, Sun Chuanqing, Fu Yongcai, Zhang Kun
Frontiers Science Center for Molecular Design Breeding (MOE), National Center for Evaluation of Agricultural Wild Plants (Rice), Department of Plant Genetics and Breeding, China Agricultural University, Beijing, China.
School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, China.
Plant Biotechnol J. 2025 Feb;23(2):615-631. doi: 10.1111/pbi.14524. Epub 2024 Nov 25.
The functional stay-green trait is a major goal of rice breeding. Here, we cloned OsSCE1a encoding SUMO-conjugating enzyme from Yuanjiang common wild rice, which simultaneously regulates the functional stay-green trait and growth duration. Low expression or knocking out OsSCE1a corresponded to increased chlorophyll content, photosynthetic competence, N use efficiency and a shortened growth period without affecting yield. A natural MITE-transposon insertion/deletion in the OsSCE1a promoter is the functional variation that regulates these traits. OsSCE1a was selected during evolution and shows significant variation between indica and japonica rice. OsNAC2 interacts with the MITE to enhance OsSCE1a expression. Genetic manipulation of OsSCE1a revealed its potential for rice improvement. OsSCE1a-mediated SUMOylation of OsGS2 suppresses GS (involved in N assimilation) enzyme activity. OsSCE1a also regulates growth duration by SUMOylating the transcription factor such as OsGBP1, which regulates the expression of the key heading gene Ghd7. Our findings shed light on the role of SUMOylation in crops and provide a strategy for increasing agricultural productivity.
功能型持绿性状是水稻育种的主要目标。在此，我们从元江普通野生稻中克隆了编码SUMO结合酶的OsSCE1a，它同时调控功能型持绿性状和生育期。OsSCE1a低表达或敲除会导致叶绿素含量增加、光合能力增强、氮利用效率提高以及生育期缩短，且不影响产量。OsSCE1a启动子中的一个天然微型反向重复转座元件（MITE）插入/缺失是调控这些性状的功能变异。OsSCE1a在进化过程中被选择，在籼稻和粳稻之间表现出显著差异。OsNAC2与该MITE相互作用以增强OsSCE1a的表达。对OsSCE1a的基因操作揭示了其在水稻改良中的潜力。OsSCE1a介导的OsGS2的SUMO化修饰抑制了GS（参与氮同化）酶的活性。OsSCE1a还通过对转录因子如OsGBP1进行SUMO化修饰来调控生育期，OsGBP1调控关键抽穗基因Ghd7的表达。我们的研究结果揭示了SUMO化修饰在作物中的作用，并为提高农业生产力提供了一种策略。
