Zhao Junqi, Zhi Yue, Ren Haozhen, Wang Jinglin, Zhao Yuanjin
Department of Hepatobiliary Surgery, Hepatobiliary Institute, Nanjing Drum Tower Hospital, Medical School, Nanjing University, Nanjing, 210008, China.
Department of Gastrointestinal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325035, China.
Bioact Mater. 2024 Nov 8;45:1-18. doi: 10.1016/j.bioactmat.2024.11.002. eCollection 2025 Mar.
The engineering construction of the liver has attracted enormous attention. Organoids, as emerging miniature three-dimensional cultivation units, hold significant potential in the biomimetic simulation of liver structure and function. Despite notable successes, organoids still face limitations such as high variability and low maturity. To overcome these challenges, engineering strategies have been established to maintain organoid stability and enhance their efficacy, laying the groundwork for the development of advanced liver organoids. The present review comprehensively summarizes the construction of engineered liver organoids and their prospective applications in biomedicine. Initially, we briefly present the latest research progress on matrix materials that maintain the three-dimensional morphology of organoids. Next, we discuss the manipulative role of engineering technologies in organoid assembly. Additionally, we outline the impact of gene-level regulation on organoid growth and development. Further, we introduce the applications of liver organoids in disease modeling, drug screening and regenerative medicine. Lastly, we overview the current obstacles and forward-looking perspectives on the future of engineered liver organoids. We anticipate that ongoing innovations in engineered liver organoids will lead to significant advancements in medical applications.
肝脏的工程构建已引起了极大关注。类器官作为新兴的微型三维培养单元，在肝脏结构和功能的仿生模拟方面具有巨大潜力。尽管取得了显著成功，但类器官仍面临诸如高变异性和低成熟度等局限性。为克服这些挑战，已制定了工程策略来维持类器官的稳定性并提高其功效，为先进肝脏类器官的发展奠定了基础。本综述全面总结了工程化肝脏类器官的构建及其在生物医学中的潜在应用。首先，我们简要介绍了维持类器官三维形态的基质材料的最新研究进展。接下来，我们讨论工程技术在类器官组装中的操纵作用。此外，我们概述了基因水平调控对类器官生长和发育的影响。进一步地，我们介绍了肝脏类器官在疾病建模、药物筛选和再生医学中的应用。最后，我们概述了工程化肝脏类器官当前面临的障碍以及对其未来的前瞻性展望。我们预计，工程化肝脏类器官的持续创新将推动医学应用取得重大进展。
