Xu Jiaying, Yuan Zeming, Zhao Huazhang, Wu Xinru, Cai Nina, Ma Tingting, Tang Bin, Chen Gongxing, Wang Shigui
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
School of Pharmacy, Hangzhou Normal University, Hangzhou 311121, China.
Insects. 2024 Nov 14;15(11):891. doi: 10.3390/insects15110891.
is a downstream target gene of cellular nutrient and growth factors, oxidative stress responses, and insulin signaling pathways. It play a crucial role in insect growth, development, and reproduction. is a significant agricultural pest; therefore, the identification of novel control targets for its management is of significant importance. After injecting dsRNA to interfere with expression, we observed changes in the reproduction-related gene expression and ovary development through RT-qPCR and morphological observation. Simultaneously, the trehalose and glycogen contents were measured following RNAi. The results demonstrate that interference with significantly downregulates key genes in the Hippo pathway and gene expression. In terms of carbohydrate metabolism, the trehalose content decreases significantly while the glycogen content increases markedly after silencing. Additionally, silencing considerably inhibits reproductive-related gene expression, resulting in delayed ovarian development. These findings indicate that regulates reproduction through the Hippo signaling pathway: when impaired, the reproductive capacity function declines. In addition, -mediated energy mobilization is involved in the regulation of egg production. These results indicate that the RNAi of may be a useful control strategy against .
是细胞营养和生长因子、氧化应激反应以及胰岛素信号通路的下游靶基因。它在昆虫生长、发育和繁殖中起着关键作用。是一种重要的农业害虫；因此，确定其防治的新靶点具有重要意义。在注射dsRNA干扰表达后，我们通过RT-qPCR和形态学观察，观察了生殖相关基因表达和卵巢发育的变化。同时，在RNA干扰后测量了海藻糖和糖原含量。结果表明，干扰显著下调了Hippo通路中的关键基因和基因表达。在碳水化合物代谢方面，沉默后海藻糖含量显著降低，而糖原含量显著增加。此外，沉默显著抑制生殖相关基因表达，导致卵巢发育延迟。这些发现表明，通过Hippo信号通路调节生殖：当受损时，生殖能力功能下降。此外，介导的能量动员参与了产卵的调节。这些结果表明，的RNA干扰可能是一种有效的防治策略。
