Department of Biotechnology, Chemistry & Pharmacy, University of Siena, Via A. Moro, 53100 Siena, Italy.
SienabioACTIVE, University of Siena, Via Aldo Moro, 53100 Siena, Italy.
Mar Drugs. 2024 Nov 13;22(11):511. doi: 10.3390/md22110511.
The circular bioeconomy is currently a promising model for repurposing natural sources; these sources include plants due to their abundance of bioactive compounds. This study evaluated the antimicrobial properties of a extract. is an invasive macroalga from the Orbetello Lagoon (Tuscany, Italy), which grows in nutrient-rich environments and has been forming extended mats since 2005. The biomass is mechanically harvested and treated as waste, consuming considerable manpower and financial resources. As a potential way to increase the value of such waste, this study found that extract (CLE) is a source of antimicrobial compounds. The phytochemical characterization of the extract revealed the predominant presence of palmitic acid, a fatty acid with known antimicrobial activity. Based on such findings, four bacterial species of high clinical relevance (, , , and ) were tested, revealing a notable antibacterial activity of the extract on (MIC, 32 μg/mL). Computational analyses identified a potential molecular target for palmitic acid, offering molecular insights on the interaction. This study presents a comprehensive in vitro and in silico approach for drug and target discovery studies by repurposing as a source of antimicrobial bioactive compounds.
循环生物经济目前是重新利用自然资源的一种有前途的模式；这些资源包括植物，因为它们含有丰富的生物活性化合物。本研究评估了一种 提取物的抗菌特性。 是一种来自奥尔贝泰洛 Lagoon（意大利托斯卡纳）的入侵性大型藻类，在富营养环境中生长，并自 2005 年以来一直形成扩展的垫子。生物质通过机械收获并作为废物处理，消耗大量的人力和财力。作为增加这种废物价值的一种潜在方法，本研究发现 提取物（CLE）是抗菌化合物的来源。提取物的植物化学特性分析表明，棕榈酸（一种具有已知抗菌活性的脂肪酸）的含量较高。基于这些发现，测试了四种具有高临床相关性的细菌（ 、 、 、和 ），结果表明提取物对 （MIC，32μg/mL）具有显著的抗菌活性。计算分析确定了棕榈酸的一个潜在 分子靶标，为相互作用提供了分子见解。本研究通过将 重新用作抗菌生物活性化合物的来源，提出了一种综合的体外和计算药物和靶标发现研究方法。
