灌注大鼠心脏中的鸟氨酸脱羧酶

Ornithine decarboxylase in perfused rat heart.

作者信息

Astancolle S, Bacciottini F, Davalli P, Piccinini G, Casti A, Corti A

出版信息

J Mol Cell Cardiol. 1986 Mar;18(3):223-30. doi: 10.1016/s0022-2828(86)80404-7.

Abstract

In the isolated perfused rat hearts, the activity of tissue ornithine decarboxylase gradually decreases over 90 min. In contrast, the activity of S-adenosylmethionine decarboxylase, lactate dehydrogenase, and glutamate-oxalacetate transaminase stays unchanged after a small decrease during the first 10 min. Ornithine decarboxylase is released from the perfused heart under conditions in which neither the lower molecular weight S-adenosylmethionine decarboxylase nor polyamines leak out. Ten minutes of ischaemia did not change the rate of release of ornithine decarboxylase. Ischaemia followed by reperfusion (20 min) increased release of ornithine decarboxylase.

摘要

在离体灌注大鼠心脏中，组织鸟氨酸脱羧酶的活性在90分钟内逐渐降低。相比之下，S-腺苷甲硫氨酸脱羧酶、乳酸脱氢酶和谷氨酸草酰乙酸转氨酶的活性在最初10分钟略有下降后保持不变。在较低分子量的S-腺苷甲硫氨酸脱羧酶和多胺均未泄漏的条件下，鸟氨酸脱羧酶从灌注心脏中释放出来。10分钟的缺血并未改变鸟氨酸脱羧酶的释放速率。缺血后再灌注（20分钟）增加了鸟氨酸脱羧酶的释放。