Maignan M F, Gulati O P
J Submicrosc Cytol. 1986 Jan;18(1):47-51.
Endothelial cells of porcine thoracic aorta were chosen as in vitro model to study the reaction of endothelial cells to injury induced by carbon tetrachloride (CCl4 0.2 microliter/ml). Lactate dehydrogenase (LDH) was used as the biochemical marker of cell injury. The changes in the morphology of the surface endothelial cells were observed by scanning electron microscopy. In addition, cell survival rate after replating cultures was evaluated. The pretreatment with Bencianol (10(-10) M-10(-7) M) produced cytoprotective effects against CCl4 induced cell injury on the above three parameters.
选用猪胸主动脉内皮细胞作为体外模型，研究内皮细胞对四氯化碳（CCl4，0.2微升/毫升）诱导损伤的反应。乳酸脱氢酶（LDH）用作细胞损伤的生化标志物。通过扫描电子显微镜观察表面内皮细胞形态的变化。此外，评估再接种培养后的细胞存活率。苯西阿诺（10^-10 M - 10^-7 M）预处理对上述三个参数的CCl4诱导的细胞损伤产生了细胞保护作用。
