Yu Xingang, Mu Xuanru, Yuan Kaijian, Wang Sifan, Li Yilong, Xu Hui, Li Qiaoyu, Zeng Wenjing, Li Zhili, Guo Jianchao, Hong Yang
School of Animal Science and Technology, Foshan University, Foshan 528231, China.
Agro-Tech Extension Center of Guangdong Province, Guangzhou 510500, China.
Animals (Basel). 2024 Nov 20;14(22):3344. doi: 10.3390/ani14223344.
spp. and are common intestinal pathogens capable of infecting both humans and animals, which lead to severe diarrhea and other intestinal diseases, posing a threat to public health. The Bamaxiang pig, a specialty of Bama Yao Autonomous County in Guangxi Province, China, is an important local breed in the regional pork market and an excellent model animal for biomedical research. Currently, no data is available on the prevalence or genotype distribution of these pathogens in Bamaxiang pigs. This study aimed to determine the prevalence and genetic characteristics of spp. and in three Bamaxiang pig farms located in Bama Yao Autonomous County, using molecular techniques based on the small subunit ribosomal RNA ( rRNA) gene fragment of spp. and the internal transcribed spacer (ITS) region of . All positive PCR products from the 311 fecal samples were sequenced to identify the species and genotypes of these organisms. The overall infection rates of spp. and were 34.08% (106/311) and 18.32% (57/311), respectively. Three subtypes of spp. were detected: ST1 ( = 8), ST3 ( = 3), and ST5 ( = 95). Among them, zoonotic ST5 was the dominant genotype, accounting for 89.62% (95/106) of strains, followed by the genotypes ST1 (7.54%, 8/106) and ST3 (2.83%, 3/106). Two genotypes of were detected: EbpC ( = 52) and CHG23 ( = 5), with EbpC being the dominant genotype. The human-pathogenic subtypes (ST1, ST3, and ST5) and genotypes (EbpC, CHG23) that were observed in this study indicate a potential threat to public health. Our findings offer basic information for preventing and controlling these zoonotic pathogens in the study area. Additional investigations are necessary to better understand their genetic characteristics and zoonotic potential within Guangxi Province.
某些物种和……是常见的肠道病原体，能够感染人类和动物，导致严重腹泻和其他肠道疾病，对公众健康构成威胁。巴马香猪是中国广西巴马瑶族自治县的特产，是当地猪肉市场的重要地方品种，也是生物医学研究的优良模式动物。目前，尚无关于这些病原体在巴马香猪中的流行情况或基因型分布的数据。本研究旨在利用基于某些物种的小亚基核糖体RNA（rRNA）基因片段和……的内部转录间隔区（ITS）区域的分子技术，确定位于巴马瑶族自治县的三个巴马香猪场中某些物种和……的流行情况及遗传特征。对311份粪便样本的所有阳性PCR产物进行测序，以鉴定这些生物体的种类和基因型。某些物种和……的总体感染率分别为34.08%（106/311）和18.32%（57/311）。检测到某些物种的三个亚型：ST1（n = 8）、ST3（n = 3）和ST5（n = 95）。其中，人畜共患的ST5是主要基因型，占菌株的89.62%（95/106），其次是基因型ST1（7.54%，8/106）和ST3（2.83%，3/106）。检测到……的两种基因型：EbpC（n = 52）和CHG23（n = 5），EbpC是主要基因型。本研究中观察到的人致病性亚型（ST1、ST3和ST5）和基因型（EbpC、CHG23）表明对公众健康存在潜在威胁。我们的研究结果为研究区域内预防和控制这些人畜共患病原体提供了基础信息。有必要进行进一步调查，以更好地了解它们在广西省内的遗传特征和人畜共患潜力。
