Environmental, Occupational, Aging (Integrative) Physiology Laboratory, Haute Ecole Bruxelles-Brabant (HE2B), 1160 Brussels, Belgium.
Anatomical Research and Clinical Studies, Vrije Universiteit Brussels (VUB), 1090 Brussels, Belgium.
Int J Mol Sci. 2024 Nov 10;25(22):12067. doi: 10.3390/ijms252212067.
The goal of this study was to evaluate inflammatory and oxidative stress responses in human subjects (9 females and 15 males) (age [29.6 ± 11.5 years old (mean ± SD)], height [172.0 ± 10.05 cm], and weight [67.8 ± 12.4 kg]) exposed to 1.45 ATA of helium (He) or nitrogen (N) without concurrent hyperoxia. We hypothesized that elevated gas pressures would elicit an inflammatory response concurrent with oxidative stress. Consistent with ex vivo studies, both gasses elicited neutrophil activation, small elevations in microparticles (MPs) and increases in intra-MP interleukin (IL)-1β and inflammatory nitric oxide synthase, and an increase in urinary IL-6 concurrent with a marked reduction in plasma gelsolin. Mixed responses indictive of oxidative stress, with some biomarker elevations but little change in others and a decrease in some, were observed. Overall, these results demonstrate that exposure to typical diving gasses at a mildly elevated partial pressure will initiate inflammatory responses, which may play a significant role in decompression sickness (DCS). The complex pattern of oxidative stress responses may be indicative of competing systemic reactions and sampling different body fluids.
本研究旨在评估人类受试者（9 名女性和 15 名男性）（年龄 [29.6 ± 11.5 岁（均值 ± 标准差）]、身高 [172.0 ± 10.05cm] 和体重 [67.8 ± 12.4kg]）在暴露于 1.45ATA 氦（He）或氮（N）而不伴有高氧时的炎症和氧化应激反应。我们假设升高的气压会引起炎症反应伴氧化应激。与离体研究一致，两种气体都引起中性粒细胞活化，微颗粒（MPs）的小幅度升高，以及 MPs 内白细胞介素（IL）-1β和炎症性一氧化氮合酶的增加，以及尿 IL-6 的增加伴随着血浆凝胶蛋白的明显减少。观察到混合反应指示氧化应激，一些生物标志物升高，但其他标志物变化不大，一些标志物下降。总的来说，这些结果表明，在轻度升高的分压下暴露于典型的潜水气体将引发炎症反应，这可能在减压病（DCS）中起重要作用。氧化应激反应的复杂模式可能表明存在竞争性的全身反应，并对不同的体液进行采样。
