CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Amyris Bio Products Portugal, Unipessoal Lda, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Int J Mol Sci. 2024 Nov 14;25(22):12224. doi: 10.3390/ijms252212224.
Cannabinoids have long been known for their bioactive properties, with their topical application as anti-inflammatory compounds being at the forefront of research for the past decade. Concurrently, the cosmetic market is a fast-growing industry in constant need of new biomolecules. In this work, we studied the safety profile for topical applications of two cannabinoids: cannabidiol (CBD) and cannabigerol (CBG) and assessed their potential as skin care ingredients. The CBG used in this work resulted from bio-fermentation, and to the best of our knowledge, there are no extensive reports on its safety and usage as a cosmetic ingredient. Our results show that CBD and CBG do not exhibit cytotoxicity, mutagenicity, or skin sensitization. Moreover, we verified an absence of primary irritability, accumulated irritability, phototoxicity and photosensitization, supporting the claims of dermatologically tested, hypoallergenic and non-irritating. While these cannabinoids did not show significant anti-aging effects by altering the extracellular matrix components (both in vitro and ex vivo), they demonstrated promise as protective agents against inflammation caused by air pollution. Specifically, they reduced the levels of pro-inflammatory cytokines, making them valuable in combating environmental skin damage. Overall, our results validate the safety of topical use of cannabinoids, while paving the way for further research in the beauty and personal care market as soothing agents.
大麻素以其生物活性特性而闻名已久，在过去十年中，其作为抗炎化合物的局部应用一直是研究的前沿。与此同时，化妆品市场是一个快速发展的行业，不断需要新的生物分子。在这项工作中，我们研究了两种大麻素：大麻二酚 (CBD) 和大麻萜酚 (CBG) 局部应用的安全性，并评估了它们作为护肤品成分的潜力。本工作中使用的 CBG 来自生物发酵，据我们所知，目前尚无关于其作为化妆品成分的安全性和使用的广泛报道。我们的研究结果表明，CBD 和 CBG 不会表现出细胞毒性、致突变性或皮肤致敏性。此外，我们还验证了不存在原发性刺激性、累积刺激性、光毒性和光致敏性，支持了经过皮肤科测试、低变应原性和非刺激性的说法。虽然这些大麻素通过改变细胞外基质成分（无论是在体外还是在体内）并没有表现出显著的抗衰老作用，但它们作为对抗空气污染引起的炎症的保护剂具有很大的潜力。具体来说，它们降低了促炎细胞因子的水平，使其成为对抗环境皮肤损伤的有价值的物质。总的来说，我们的研究结果验证了大麻素局部使用的安全性，同时为其在美容和个人护理市场中作为舒缓剂的进一步研究铺平了道路。
