评估哈萨克斯坦的 Regel 和 Regel 物种的完整质体基因组序列。

Assessment of Complete Plastid Genome Sequences of Regel and Regel Species from Kazakhstan.

机构信息

Institute of Plant Biology and Biotechnology, Almaty 050040, Kazakhstan.

Institute of Zoology, Almaty 050060, Kazakhstan.

出版信息

Genes (Basel). 2024 Nov 9;15(11):1447. doi: 10.3390/genes15111447.

DOI:10.3390/genes15111447

PMID:39596647

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11593697/

Abstract

BACKGROUND

species are economically, culturally, scientifically, and ecologically important. Tulips present taxonomic complexities that cannot be adequately resolved by examining their morphological characteristics alone or by relying on a limited selection of genetic markers.

METHODS

In the present study, we assessed the complete plastid sequences of Regel and Regel collected from Kazakhstan. Additionally, 14 previously published plastomes were obtained from GenBank for comparison and phylogenetic analysis.

RESULTS

The plastid genome sizes of and were 152,359 bp and 152,242 bp, respectively. In the plastid genomes of and , 136 genes were annotated, 114 of which were unique. These unique genes comprised eighty protein-coding, thirty transfer RNA, and four ribosomal RNA genes. Additionally, 415 simple sequence repeats were identified, comprising 107 tandem, 40 forward, 49 palindromic, 8 reverse, and 1 complementary repeat. Notably, the region containing exhibited high variability and may serve as an informative DNA barcode for this genus.

CONCLUSION

Phylogenetic analysis showed strong support for the relationships among species, indicating the utility of plastid genome data for further taxonomic studies within the genus.

摘要

背景

物种在经济、文化、科学和生态方面都具有重要意义。郁金香具有分类学上的复杂性，仅通过检查其形态特征或仅依赖有限的遗传标记选择无法充分解决。

方法

本研究评估了来自哈萨克斯坦的 Regel 和 Regel 的完整质体序列。此外，从 GenBank 获得了 14 个先前发表的质体基因组，用于比较和系统发育分析。

结果

和的质体基因组大小分别为 152359bp 和 152242bp。在和的质体基因组中，注释了 136 个基因，其中 114 个是独特的。这些独特的基因包括 80 个蛋白质编码基因、30 个转移 RNA 基因和 4 个核糖体 RNA 基因。此外，还鉴定了 415 个简单序列重复，包括 107 个串联重复、40 个正向重复、49 个回文重复、8 个反向重复和 1 个互补重复。值得注意的是，包含的区域表现出高度的变异性，可能作为该属的有价值 DNA 条形码。