Gutiérrez-Fernández José, Cerezo-Collado Laura, Garcés Víctor, Alarcón-Guijo Pablo, Delgado-López José M, Dominguez-Vera Jose M
Department of Microbiology, Virgen de las Nieves University Hospital, 18014 Granada, Spain.
Departmento de Química Inorgánica, Instituto de Biotecnología, Universidad de Granada. 18071 Granada, Spain.
Antibiotics (Basel). 2024 Oct 25;13(11):1003. doi: 10.3390/antibiotics13111003.
Carbapenems are one of the mainstays of treatment for antibiotic-resistant bacteria (ARB). This has made the rise of carbapenem-resistant bacteria a threat to global health. In fact, the World Health Organization (WHO) has identified carbapenem-resistant bacteria as critical pathogens, and the development of novel antibacterials capable of combating infections caused by these bacteria is a priority. : With the aim of finding new alternatives to fight against ARB and especially against carbapenem-resistant bacteria, we have developed a series of living materials formed by incorporating the probiotics (), (), and a mixture of both (+) into bacterial cellulose (BC). : These probiotic-loaded bacterial celluloses inhibited the proliferation of three ARB, including two carbapenem-resistant enterobacteria (CRE), identified as and , and a carbapenem-resistant . Interestingly, while the probiotics , , and the mixture of both were found to be inactive against these ARB, they became active once incorporated into BC. : The increase in activity is due to the known effect that cells increase their activity once incorporated into a suitable matrix, forming a living material. For the same reasons, the probiotics in the living materials BC-, BC-, and BC-+ showed increased stability, allowing them to be stored with bacterial activity for long periods of time (two months).
碳青霉烯类药物是治疗耐抗生素细菌（ARB）的主要手段之一。这使得耐碳青霉烯类细菌的出现对全球健康构成了威胁。事实上，世界卫生组织（WHO）已将耐碳青霉烯类细菌确定为关键病原体，开发能够对抗由这些细菌引起的感染的新型抗菌药物是当务之急。：为了寻找对抗ARB尤其是耐碳青霉烯类细菌的新替代方法，我们开发了一系列通过将益生菌（）、（）以及两者的混合物（+）掺入细菌纤维素（BC）中形成的活性材料。：这些负载益生菌的细菌纤维素抑制了三种ARB的增殖，其中包括两种耐碳青霉烯类肠杆菌（CRE），分别鉴定为和，以及一种耐碳青霉烯类。有趣的是，虽然发现益生菌、以及两者的混合物对这些ARB无活性，但一旦掺入BC中它们就变得有活性了。：活性的增加归因于细胞一旦掺入合适的基质中形成活性材料就会增加其活性这一已知效应。出于同样的原因，活性材料BC - 、BC - 和BC - +中的益生菌显示出更高的稳定性，使其能够在保持细菌活性的状态下长时间储存（两个月）。
