Tăbăran Alexandra, Dan Sorin Danel, Colobaţiu Liora Mihaela, Mihaiu Marian, Condor Sergiu, Mărgăoan Rodica, Crişan-Reget Oana Lucia
Department of Animal Husbandry and Public Health, Faculty of Veterinary Medicine Cluj-Napoca, University of Agricultural Sciences and Veterinary Medicine Cluj, 400372 Cluj-Napoca, Romania.
Department of Medical Devices, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, Victor Babes Street No. 8, 400012 Cluj-Napoca, Romania.
Microorganisms. 2024 Oct 30;12(11):2196. doi: 10.3390/microorganisms12112196.
Antibiotic resistance among pathogenic bacteria in humans and animals poses a critical public health challenge, leading to diminished effectiveness of existing antimicrobial treatments. Notably, animal-derived food products are significant vectors for the transmission of resistant bacteria to humans, with species being predominant culprits in foodborne illnesses. This study investigates the prevalence and antibiotic resistance patterns of serovars isolated from traditionally sourced pork meat in Romania. Over a four-year period, 208 pork samples were collected using standardized protocols during traditional slaughtering practices. spp. were isolated following ISO 6579:2002 guidelines and confirmed using biochemical assays and PCR. Serotyping was performed using specific antisera, and antimicrobial susceptibility testing was conducted through the standard disk diffusion method, assessing 11 antibiotics. Results indicated a 23.07% prevalence of Salmonella, with 48 isolates categorized into eight serovars, primarily . Infantis ( = 15), . Typhimurium ( = 15), and . Derby ( = 11). PCR results confirmed the presence of by detecting the and genes, with 31.25% of isolates being positive for the -specific sequence. Notably, 93.75% of the isolates were multidrug-resistant (MDR), exhibiting high resistance rates against streptomycin (91.66%) (>10 µg), tetracycline (83.33%) (>30 µg), and sulfamethoxazole (68.75%) (>300 µg). More than 60% of MDR isolates displayed resistance to five or more antibiotics. These findings underscore the need for coordinated control measures in the pork production chain to combat the spread of and protect public health. Enhanced surveillance and intervention strategies are crucial for addressing antibiotic resistance and reducing the risk of foodborne illnesses linked to contaminated animal products.
人类和动物体内病原菌的抗生素耐药性构成了严峻的公共卫生挑战，导致现有抗菌治疗的效果降低。值得注意的是，动物源食品是耐药菌向人类传播的重要载体，某些菌种是食源性疾病的主要罪魁祸首。本研究调查了从罗马尼亚传统来源猪肉中分离出的血清型的流行情况和抗生素耐药模式。在四年时间里，在传统屠宰过程中使用标准化方案收集了208份猪肉样本。按照ISO 6579:2002指南分离出沙门氏菌属，并通过生化分析和PCR进行确认。使用特异性抗血清进行血清分型，并通过标准纸片扩散法进行抗菌药敏试验，评估11种抗生素。结果表明，沙门氏菌的流行率为23.07%，48株分离株分为8个血清型，主要是婴儿沙门氏菌（=15）、鼠伤寒沙门氏菌（=15）和德比沙门氏菌（=11）。PCR结果通过检测invA和spvC基因证实了沙门氏菌的存在，31.25%的分离株invA特异性序列呈阳性。值得注意的是，93.75%的分离株对多种药物耐药（MDR），对链霉素（91.66%）（>10μg）、四环素（83.33%）（>30μg）和磺胺甲恶唑（68.75%）（>300μg）表现出高耐药率。超过60%的MDR分离株对五种或更多抗生素耐药。这些发现强调了在猪肉生产链中采取协调控制措施以对抗沙门氏菌传播和保护公众健康的必要性。加强监测和干预策略对于应对抗生素耐药性和降低与受污染动物产品相关的食源性疾病风险至关重要。
