Prevalence and antimicrobial resistance of serovars Enteritidis and Typhimurium isolated from retail chicken meat in Wasit markets, Iraq.

BACKGROUND AND AIM

Food poisoning caused by serovars is the most common type of foodborne illness. Tainted chicken meat is a major vector for spreading these serovars throughout the food supply chain. isolates that developed resistance to commonly used antimicrobials pose a noteworthy risk to public health, yet there has been a lack of data on this issue in Iraq. Therefore, it is crucial to address these serious public health challenges with an adequate database on the occurrence and antibiotic resistance of these serovars. This study aimed to determine the frequency of occurrence of serovars Enteritidis and Typhimurium (. Enteritidis and . Typhimurium), antimicrobial resistance (AMR), and prevalence of multidrug resistance among . Enteritidis and . Typhimurium isolated from poultry meat collected in Wasit Province in Iraq.

MATERIALS AND METHODS

A total of 150 raw and frozen poultry meat samples were gathered from retail markets in various locales across the Wasit Governorate in Iraq. spp. were successfully cultured and identified using the technique recommended by ISO 6579:2002, with minor modifications. The multiplex polymerase chain reaction approach was used to confirm spp. (. Enteritidis and . Typhimurium). A disk diffusion test was performed to determine the susceptibility to particular antimicrobial agents, and 12 different antimicrobial agents were evaluated.

RESULTS

Only 19 of the 150 (12.7%) samples tested positive for (16% and 11% were isolated from raw and frozen chicken meat, respectively). . Enteritidis accounted for 63.2%, whereas . Typhimurium accounted for 36.8%. Nalidixic acid resistance was the most common (73.7%), followed by sulfamethoxazole-trimethoprim (63.2%) and tetracycline (63.2%), but gentamicin and ciprofloxacin (up to 15.8%) only had modest resistance. Antibiogram of . Enteritidis and . Typhimurium yield 13 antibiotypes. Among the 19 isolates, 12 of 19 (63.2%) established resistance to no less than three categories of antimicrobials.

CONCLUSION

This study highlighted the necessity of limiting the utilization of antibiotics in animal production by providing vital information regarding the frequency and AMR of at markets in Wasit Province. Therefore, risk assessment models could use these data to lessen the amount of passed on to humans in Iraq from chicken meat.