Li Jindou, Ding Jiaxin, Guo Chunhong, Xu Xiaohong, Shan Chunhui, Qian Jing, Ding Zhuang
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Microorganisms. 2024 Nov 8;12(11):2266. doi: 10.3390/microorganisms12112266.
Goose-derived Newcastle disease (ND) and gosling plague (GP) are serious threats to the goose industry. Conventional vaccines have made significant contributions to preventing GP and ND. Nevertheless, the renewal of conventional vaccines and the application of novel vaccines are urgently needed to align with eco-friendly and efficient breeding concepts and achieve the final goal of epidemic purification. Therefore, based on the Newcastle disease virus-like particles (ND VLPs) vector platform, we developed novel chimeric ND-GP bivalent cVLPs (ND-GP cVLPs) displaying the NDV HN protein and the GPV VP3 protein. In vivo, immunization experiments revealed that geese immunized with 30 µg, 50 µg, or 70 µg of the ND-GP cVLPs and commercial vaccines produced highly effective hemagglutination inhibitory antibodies against NDV and neutralizing antibodies against GPV, respectively. Furthermore, 70 µg of the ND-GP cVLPs effectively protected against virulent NDV and GPV, reducing tissue damage from viral infection and virus shedding in the oropharynx and cloaca. In conclusion, we provide eco-friendly and efficient novel ND-GP cVLPs for preventing goose-derived ND and GP. Our findings provide the basis for using ND VLPs as foreign protein carriers for the developing of multi-conjugate vaccines.
鹅源新城疫（ND）和小鹅瘟（GP）对养鹅业构成严重威胁。传统疫苗在预防GP和ND方面做出了重大贡献。然而，迫切需要更新传统疫苗并应用新型疫苗，以符合生态友好和高效养殖理念，并实现疫病净化的最终目标。因此，基于新城疫病毒样颗粒（ND VLPs）载体平台，我们开发了新型嵌合ND-GP二价cVLPs（ND-GP cVLPs），其展示了新城疫病毒（NDV）血凝素神经氨酸酶（HN）蛋白和鹅细小病毒（GPV）病毒蛋白3（VP3）。在体内，免疫实验表明，用30 μg、50 μg或70 μg的ND-GP cVLPs和商业疫苗免疫的鹅分别产生了针对NDV的高效血凝抑制抗体和针对GPV的中和抗体。此外，70 μg的ND-GP cVLPs有效地抵御了强毒NDV和GPV，减少了病毒感染造成的组织损伤以及口咽和泄殖腔中的病毒 shedding。总之，我们提供了生态友好且高效的新型ND-GP cVLPs用于预防鹅源ND和GP。我们的研究结果为将ND VLPs用作开发多共轭疫苗的外源蛋白载体提供了依据。
