Parish E J, Nanduri V B, Kohl H H, Taylor F R
Lipids. 1986 Jan;21(1):27-30. doi: 10.1007/BF02534299.
As a class of compounds, oxysterols have demonstrated a wide variety of biological properties. Due to the general interest in these compounds, new methods of chemical synthesis have been developed to provide them for biological investigation. The specific inhibition by oxysterols of cholesterol biosynthesis in mammalian cells has been shown to result primarily from a decrease in cellular levels of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity. Recent evidence suggests these cellular responses may be mediated by an oxysterol binding protein found in the cytosol of many lines of cultured cells. In certain instances, oxysterols have been shown to be produced in biological systems. These results support the supposition that oxysterols may regulate sterol biosynthesis at the cellular level. Included herein are the inhibitory effects of 9 alpha, 11 alpha-epoxycholest-7-en-3 beta-ol cholest-8-en-3 beta-ol-7-one and cholest-8-en-3 beta-ol-11-one on HMG-CoA reductase activity and their relative affinities for a cytosolic binding protein.
作为一类化合物，氧化甾醇已显示出多种多样的生物学特性。由于对这些化合物的普遍关注，已开发出新的化学合成方法来制备它们用于生物学研究。已表明氧化甾醇对哺乳动物细胞中胆固醇生物合成的特异性抑制主要是由于细胞内3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶活性水平降低所致。最近的证据表明，这些细胞反应可能由许多培养细胞系的细胞质中发现的一种氧化甾醇结合蛋白介导。在某些情况下，已证明氧化甾醇在生物系统中产生。这些结果支持氧化甾醇可能在细胞水平调节甾醇生物合成的推测。本文包括9α,11α-环氧胆甾-7-烯-3β-醇、胆甾-8-烯-3β-醇-7-酮和胆甾-8-烯-3β-醇-11-酮对HMG-CoA还原酶活性的抑制作用及其对细胞质结合蛋白的相对亲和力。
