Clark-Pérez Diana Laura, Romo-Sáenz César Iván, Ramírez-Villalobos Jesica María, Tamez-Guerra Patricia, Caballero-Hernández Diana, Delgado-Miranda Ana Laura, García Andrés, Elizondo-Luevano Joel Horacio, Rodríguez-Padilla Cristina, Gomez-Flores Ricardo
Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza 66455, Nuevo León, Mexico.
Universidad Autónoma del Estado de Morelos Centro de Investigación en Biotecnología, Laboratorio de Biotecnología Ambiental, Av. Universidad 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, Mexico.
Microorganisms. 2024 Nov 13;12(11):2310. doi: 10.3390/microorganisms12112310.
Cancer belongs to a group of diseases characterized by uncontrolled cell growth. The search for new effective treatments for cancer has led to the discovery of different molecules from plants, bacteria, and fungi with pharmacological use. Plant endophytic fungi are large producers of metabolites with antitumor properties. We aimed to evaluate the in vitro and in vivo antitumor potential of extracts from endophytic fungi. We obtained ethyl acetate and hexane extracts from the endophytes and and evaluated their antitumor activity against murine L5178Y-R lymphoma cells and human peripheral blood mononuclear cells, using the 3-(4,5-dimethylthiazol-2-yl)-2-diphenyltetrazolium bromide reduction colorimetric technique. and ethyl acetate extracts showed IC values of 9.168 ± 1.21 μg/mL and 13.51 ± 1.62, respectively, and selectivity indices > 30. We also observed that the maximum tolerated dose (100 mg/kg) of ethyl acetate extracts and the vehicle in BALB/c mice did not cause hepatotoxicity. In addition, we evaluated the effects of ethyl acetate extracts on survival and tumor volume in the L5178Y-R lymphoma tumor model. An increase in survival (17 d) was observed in mice treated with extract. Furthermore, it did not increase tumor volume during 10 d, as compared with the control groups without treatment, vehicle, and extract, which had a maximum survival of 10 d. ethyl acetate extract showed in vitro and in vivo antitumor activity against lymphoma L5178Y-R, increasing mice survival.
癌症属于一组以细胞生长失控为特征的疾病。对癌症新的有效治疗方法的探索导致了从植物、细菌和真菌中发现了具有药理学用途的不同分子。植物内生真菌是具有抗肿瘤特性的代谢产物的大量生产者。我们旨在评估内生真菌提取物的体外和体内抗肿瘤潜力。我们从内生真菌中获得了乙酸乙酯和己烷提取物，并使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐还原比色法评估了它们对小鼠L5178Y-R淋巴瘤细胞和人外周血单个核细胞的抗肿瘤活性。乙酸乙酯提取物的IC值分别为9.168±1.21μg/mL和13.51±1.62，选择性指数>30。我们还观察到，乙酸乙酯提取物和载体在BALB/c小鼠中的最大耐受剂量（100mg/kg）不会引起肝毒性。此外，我们评估了乙酸乙酯提取物对L5178Y-R淋巴瘤肿瘤模型中生存和肿瘤体积的影响。在用提取物处理的小鼠中观察到生存期延长（17天）。此外，与未处理的对照组、载体组和提取物组相比，其在10天内肿瘤体积没有增加，未处理的对照组、载体组和提取物组的最大生存期为10天。乙酸乙酯提取物对淋巴瘤L5178Y-R显示出体外和体内抗肿瘤活性，延长了小鼠生存期。
