Ramírez-Villalobos Jesica María, Romo-Sáenz César Iván, Quintanilla-Licea Ramiro, Tamez-Guerra Patricia, Pérez-González Orquídea, Estupiñan-Jiménez José Roberto, Torres-Hernández Ángel David, Rodríguez-Garza Nancy Edith, Rodríguez-Padilla Cristina, Gomez-Flores Ricardo
Campus Cumbres, Universidad del Valle de México, Monterrey, Mexico.
Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
Int J Microbiol. 2025 Mar 12;2025:1349429. doi: 10.1155/ijm/1349429. eCollection 2025.
Identification of new sources of compounds with anticancer and antioxidant potential is essential for cancer treatment. Rhizospheric bacteria have emerged as a promising source of these agents. This study is aimed at investigating the anticancer and antioxidant properties of bacteria isolated from the rhizosphere of . Six rhizobacteria of were isolated and molecularly identified by sequencing the 16S rRNA gene and MALDI-TOF. We evaluated the inhibitory activity of methanol (MeOH) and ethyl acetate (EtOAc) extracts obtained from rhizobacteria against the murine lymphoma cell line L5178Y-R, as well as normal Vero monkey kidney cells and peripheral blood mononuclear cells (PBMCs). Compared with those of normal cells, the MeOH extract of sp. PMS-B023 showed selective anticancer activity against lymphoma, with 50% inhibitory concentration (IC) values of 117.5 g/mL and a selectivity index (SI) > 2.1, as compared with those of normal cells. L-Pro-L-Val cycle, gageostatin B, cinnamic acid, parvifloracin, daidzein, genistein, actinomycin D, actinomycin Y4, and surfactin C were detected in the MeOH extract, which may be responsible for its antilymphoma activity. We did not find significant antioxidant activity in any of the extracts evaluated using the 2,2-diphenyl-1-picrylhydrazyl technique (IC > 250 g/mL). The results showed that sp. PMS-B023 has antitumor potential and selective activity against L5178Y-R lymphoma cells. The presence of anticancer compounds was also demonstrated, as previously described in the literature.
鉴定具有抗癌和抗氧化潜力的新化合物来源对于癌症治疗至关重要。根际细菌已成为这些药物的一个有前景的来源。本研究旨在调查从[具体植物名称]根际分离的细菌的抗癌和抗氧化特性。通过对16S rRNA基因进行测序和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）对分离自[具体植物名称]根际的六种根际细菌进行了分子鉴定。我们评估了从根际细菌中获得的甲醇（MeOH）和乙酸乙酯（EtOAc）提取物对小鼠淋巴瘤细胞系L5178Y-R以及正常的非洲绿猴肾细胞和外周血单个核细胞（PBMC）的抑制活性。与正常细胞相比，[具体菌株名称]sp. PMS-B023的MeOH提取物对淋巴瘤显示出选择性抗癌活性，其50%抑制浓度（IC）值为117.5μg/mL，与正常细胞相比，选择性指数（SI）>2.1。在MeOH提取物中检测到L-脯氨酸-L-缬氨酸循环、gageostatin B、肉桂酸、小花黄堇素、大豆苷元、染料木黄酮、放线菌素D、放线菌素Y4和表面活性素C，这可能是其抗淋巴瘤活性的原因。使用2,2-二苯基-1-苦基肼技术评估的任何提取物中均未发现显著的抗氧化活性（IC>250μg/mL）。结果表明，[具体菌株名称]sp. PMS-B023具有抗肿瘤潜力和对L5178Y-R淋巴瘤细胞的选择性活性。如先前文献中所述，抗癌化合物的存在也得到了证实。
