Thongwattana Tiarpa, Chaiyo Ronnakorn, Ponsanti Khanittha, Tangnorawich Benchamaporn, Pratumpong Patcharee, Toommee Surachet, Jenjob Ratchapol, Yang Su-Geun, Parcharoen Yardnapar, Natphopsuk Sitakan, Pechyen Chiravoot
Department of Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Pathum Thani 12120, Thailand.
Department of Physics, Faculty of Science and Technology, Thammasat University, Pathum Thani 12120, Thailand.
Pharmaceuticals (Basel). 2024 Oct 24;17(11):1421. doi: 10.3390/ph17111421.
This study aims to develop a screen-printed carbon electrode (SPCE) modified with silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) for the detection of human serum albumin (HSA). The objectives include utilizing green synthesis methods for nanoparticle production and evaluating the electrochemical performance of the modified electrodes. AgNPs and AuNPs were synthesized using Phulae pineapple peel extract (PPA) as a reducing agent. The nanoparticles were characterized using UV-visible spectrophotometry (UV-vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM). The electrochemical performance of AgNP/SPCE and AuNP/SPCE was assessed by cyclic voltammetry (CV) studies, and the electrodes were functionalized with anti-HSA antibodies for HSA detection. Characterization revealed spherical nanoparticles ranging from 10 to 30 nm. Both AgNP/SPCE and AuNP/SPCE demonstrated improved electrochemical performance compared to bare SPCEs. The modified sensors could detect serum albumin concentrations from 10 to 400 μg/mL, with high correlation values of 0.97 and 0.99 for AgNPs and AuNPs, respectively. This research demonstrates the potential of using agricultural waste for green synthesis of nanoparticles and highlights the application of AgNPs and AuNPs in developing sensitive biosensing platforms for the detection of human serum albumin.
本研究旨在开发一种用银纳米颗粒（AgNPs）和金纳米颗粒（AuNPs）修饰的丝网印刷碳电极（SPCE），用于检测人血清白蛋白（HSA）。目标包括利用绿色合成方法制备纳米颗粒，并评估修饰电极的电化学性能。使用Phulae菠萝皮提取物（PPA）作为还原剂合成AgNPs和AuNPs。通过紫外可见分光光度法（UV-vis）、傅里叶变换红外光谱法（FTIR）、X射线衍射法（XRD）和透射电子显微镜法（TEM）对纳米颗粒进行表征。通过循环伏安法（CV）研究评估AgNP/SPCE和AuNP/SPCE的电化学性能，并将电极用抗HSA抗体功能化以检测HSA。表征显示纳米颗粒呈球形，尺寸范围为10至30nm。与裸SPCE相比，AgNP/SPCE和AuNP/SPCE均表现出改善的电化学性能。修饰后的传感器能够检测浓度为10至400μg/mL的血清白蛋白，AgNPs和AuNPs的相关值分别高达0.97和0.99。本研究证明了利用农业废弃物进行纳米颗粒绿色合成的潜力，并突出了AgNPs和AuNPs在开发用于检测人血清白蛋白的灵敏生物传感平台中的应用。
