Chemistry Graduate Program, Federal University of Pará, Rua Augusto Corrêa, 01, Belém 66075110, Brazil.
Oncology Research Center, Federal University of Pará, Rua dos Mundurucus, 4487, Belém 66073005, Brazil.
Molecules. 2024 Nov 8;29(22):5278. doi: 10.3390/molecules29225278.
The search for bioactive compounds for the treatment of several diseases has led to the study of endophytic fungi. Neoplastic diseases are among the most significant health concerns due to their high mortality rate, and there is a dearth of efficacious pharmaceutical agents for the treatment of cancer. Gastric cancer is one of the most aggressive forms of cancer and is among those with the highest mortality rates in Brazil. Accordingly, the objective of this study was to identify compounds with cytotoxic activity from the mangrove-derived endophytic fungus sp. Isolation of the chemical compounds was conducted using chromatographic methods, while structural elucidation was achieved through the application of spectroscopic (NMR and UV) and spectrometric (MS) techniques. The fungus sp. was found to produce five distinct koninginins (A, B, C, E, and J). The organic phases of the extracts and isolated compounds were evaluated for their antimicrobial and cytotoxic potentials, respectively, through microdilution testing and the MTT method. In the cytotoxicity assay, both the AF extract and koninginin A demonstrated favorable outcomes, indicating their potential as promising anticancer therapeutic agents.
为了治疗多种疾病而寻找具有生物活性的化合物，促使人们对内生真菌展开了研究。由于肿瘤疾病具有很高的死亡率，因此成为了最严重的健康问题之一，而针对癌症的有效药物制剂却十分匮乏。胃癌是最具侵袭性的癌症之一，也是巴西死亡率最高的癌症之一。因此，本研究的目的是从红树林内生真菌 sp. 中鉴定具有细胞毒性的化合物。采用色谱方法进行化合物的分离，通过光谱（NMR 和 UV）和光谱（MS）技术实现结构解析。研究发现 sp. 真菌能够产生五种独特的海绵素（A、B、C、E 和 J）。通过微量稀释试验和 MTT 法，分别对提取物和分离化合物的有机相进行了抗菌和细胞毒性评估。在细胞毒性测定中，AF 提取物和海绵素 A 都表现出了良好的效果，表明它们具有作为有前途的抗癌治疗剂的潜力。
