Ib-AMP4抗菌肽用于治疗耐甲氧西林金黄色葡萄球菌（MRSA）引起的皮肤和全身感染。
Ib-AMP4 antimicrobial peptide as a treatment for skin and systematic infection of methicillin-resistant (MRSA).
作者信息
Sadelaji Samira, Ghaznavi-Rad Ehsanollah, Sadoogh Abbasian Shabnam, Fahimirad Shohreh, Abtahi Hamid
机构信息
Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran.
Department of Medical Laboratory Sciences, Arak School of Paramedicine, Arak University of Medical Sciences, Arak, Iran.
出版信息
Iran J Basic Med Sci. 2022 Feb;25(2):232-238. doi: 10.22038/IJBMS.2022.61043.13508.
OBJECTIVES
Antimicrobial peptide compounds (AMPs) play important roles in the immune system. They also exhibit significant anti-tumor and antibacterial properties. Most AMPs are cationic and are able to bind bacterial cell membranes through electrostatic affinity. Ib-AMP4 is a plant-derived AMP that exerts rapid bactericidal functions. In the present study, the antibacterial efficiency of the produced recombinant Ib-AMP4 in elimination of Methicillin-resistant () bacterial infection, was investigated under and situations.
MATERIALS AND METHODS
The synthesized codon-optimized gene sequences of the Ib-AMP4 were expressed in . The recombinant Ib-AMP4 was purified and refolding conditions were optimized. The antibacterial efficiency of the refolded peptide against was tested under and situations for treatment of skin and systematic infection of in a mouse model.
RESULTS
Antibacterial assays confirmed the antibacterial function of Ib-AMP4 against . SEM results proved the destructive effects of applying Ib-AMP4 on biomembrane. Time-kill curve and growth kinetic assay illustrated rapid antibacterial activity of the produced Ib-AMP4. Moreover, Ib-AMP4 showed significant infection treatment ability in a mouse model and all infected mice receiving Ib-AMP4 protein survived and there was no trace of bacteria in their blood samples.
CONCLUSION
The results confirmed the rapid antibacterial potential of the produced recombinant Ib-AMP4 to be used for efficient treatment of infection.
目的
抗菌肽化合物（AMPs）在免疫系统中发挥重要作用。它们还具有显著的抗肿瘤和抗菌特性。大多数AMPs是阳离子性的，能够通过静电亲和力与细菌细胞膜结合。Ib-AMP4是一种植物源AMPs，具有快速杀菌功能。在本研究中，研究了所产生的重组Ib-AMP4在体外和体内情况下消除耐甲氧西林金黄色葡萄球菌感染的抗菌效率。
材料与方法
合成的Ib-AMP4密码子优化基因序列在大肠杆菌中表达。对重组Ib-AMP4进行纯化，并优化复性条件。在体外和体内情况下，在小鼠模型中测试复性后的肽对耐甲氧西林金黄色葡萄球菌的抗菌效率，以治疗皮肤和全身感染。
结果
抗菌试验证实了Ib-AMP4对耐甲氧西林金黄色葡萄球菌的抗菌功能。扫描电镜结果证明了应用Ib-AMP4对耐甲氧西林金黄色葡萄球菌生物膜的破坏作用。时间-杀菌曲线和生长动力学分析表明所产生的Ib-AMP4具有快速抗菌活性。此外，Ib-AMP4在小鼠模型中显示出显著的感染治疗能力，所有接受Ib-AMP4蛋白的感染小鼠均存活，且其血液样本中无细菌踪迹。
结论
结果证实了所产生的重组Ib-AMP4具有快速抗菌潜力，可用于有效治疗耐甲氧西林金黄色葡萄球菌感染。
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