Di Rosa Emilien, Durand Audrey-Anne, Provost Caroline, Constant Philippe
Centre Armand-Frappier Santé Biotechnologie, Institut national de la recherche scientifique, Laval, QC H7V 1B7, Canada.
Centre de recherche agroalimentaire de Mirabel, Mirabel, QC J7N 2X8, Canada.
Plant Dis. 2025 Mar;109(3):633-637. doi: 10.1094/PDIS-09-24-1873-RE. Epub 2025 Mar 12.
The tomato brown rugose fruit virus (ToBRFV) poses a considerable threat to tomato production worldwide. Substantial experimental evidence supports the role of infected seeds as a contamination route, but the epidemiologic portrait of the virus has received less attention. This study reports the first survey of ToBRFV prevalence in commercial greenhouses. The aim was to examine the distribution of the virus in relation to greenhouse size and management practices in Québec (Canada). Plant samples collected at three production stages in 31 commercial greenhouses were subjected to ToBRFV detection and genome sequencing. The virus was detected in seven commercial greenhouses (11 positive samples out of 311 analyzed). Retrieved partial genome sequences formed a cluster with ToBRFV variants from Canada and Mexico, suggesting cross-border propagation through commercial trades. There was no link between greenhouse features and ToBRFV diagnosis, indicating that no specific profile is more susceptible to infection than others.
番茄褐色皱纹果病毒（ToBRFV）对全球番茄生产构成了相当大的威胁。大量实验证据支持受感染种子作为一种污染途径所起的作用，但该病毒的流行病学特征却较少受到关注。本研究报告了对商业温室中ToBRFV流行情况的首次调查。目的是研究该病毒在加拿大魁北克省与温室大小及管理措施相关的分布情况。在31个商业温室的三个生产阶段采集的植物样本进行了ToBRFV检测和基因组测序。在7个商业温室中检测到了该病毒（311份分析样本中有11份呈阳性）。检索到的部分基因组序列与来自加拿大和墨西哥的ToBRFV变体形成了一个簇，表明该病毒通过商业贸易跨境传播。温室特征与ToBRFV诊断之间没有关联，这表明没有哪种特定类型的温室比其他温室更容易感染该病毒。
