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使用水性CsPbBr钙钛矿量子点的机器学习驱动荧光传感器阵列用于快速检测和杀灭食源性病原体。

Machine learning-driven fluorescent sensor array using aqueous CsPbBr perovskite quantum dots for rapid detection and sterilization of foodborne pathogens.

作者信息

Zhang Shanting, Zhu WeiWei, Zhang Xin, Mei LiangHui, Liu Jian, Wang Fangbin

机构信息

Hefei University of Technology, Hefei 230009, China.

Hefei University of Technology, Hefei 230009, China.

出版信息

J Hazard Mater. 2025 Feb 5;483:136655. doi: 10.1016/j.jhazmat.2024.136655. Epub 2024 Nov 24.

DOI:10.1016/j.jhazmat.2024.136655
PMID:39603133
Abstract

With the growing global concern over food safety, the rapid detection and disinfection of foodborne pathogens have become critical in public health. This study presents a novel machine learning-driven fluorescent sensor array utilizing aqueous CsPbBr perovskite quantum dots (PQDs) for the rapid identification and eradication of foodborne pathogens. The relative signal intensity changes (ΔRGB) generated by the sensor array were analyzed using the machine learning algorithm-Support Vector Machine (SVM). The study achieved the identification and recognition of five pathogens and their mixtures within a concentration range of 1.0 × 10 to 1.0 × 10 CFU/mL with an accuracy rate of 100 %, and the limits of detection (LOD) for the pathogens were found to be low. Additionally, the array also showed excellent performance in the identification of pathogens in tap water, achieving an accuracy rate of 100 %. Furthermore, the fluorescent sensor array was capable of inactivating the pathogens with an efficiency of over 99 % within 30 min post-detection. This development provides an efficient and reliable tool for the field of food safety detection.

摘要

随着全球对食品安全的关注度不断提高,食源性病原体的快速检测和消毒已成为公共卫生领域的关键问题。本研究提出了一种新型的机器学习驱动的荧光传感器阵列,该阵列利用水性CsPbBr钙钛矿量子点(PQDs)来快速识别和根除食源性病原体。使用机器学习算法——支持向量机(SVM)分析传感器阵列产生的相对信号强度变化(ΔRGB)。该研究实现了在1.0×10至1.0×10 CFU/mL的浓度范围内对五种病原体及其混合物的识别和鉴定,准确率达100%,且发现病原体的检测限较低。此外,该阵列在识别自来水中的病原体方面也表现出优异的性能,准确率达100%。此外,荧光传感器阵列能够在检测后30分钟内将病原体灭活,灭活效率超过99%。这一进展为食品安全检测领域提供了一种高效可靠的工具。

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