Zhang Yi, Fardous Jannatul, Zhou Yu, Wu Lichun, Doi Ryota, Hu Jia, Hirota Yuya, Ikegami Yasuhiro, Aishima Shinichi, Ijima Hiroyuki
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Department of Pharmacy, Faculty of Science, Comilla University, Cumilla 3506, Bangladesh.
J Biosci Bioeng. 2025 Feb;139(2):112-122. doi: 10.1016/j.jbiosc.2024.10.011. Epub 2024 Nov 27.
Healing diabetic foot ulcers (DFUs) poses a serious challenge for many individuals with diabetes. The use of biomaterials applied locally for treating DFUs has recently garnered significant attention. Here, we present a gel-in-oil nanogel dispersion (G/O-NGD) capable of local delivery of six different growth factors (GFs) via the topical route, followed by an in-vivo evaluation in mice. Both macroscopic and microscopic changes in skin structure were evidented after topical application of GF-cocktail G/O-NGD, and changes in CD68 and CD31 levels and collagen content were measured. Expression and synthesis of Interleukin-6 (IL-6), transforming GF beta 1 (TGF-β1), and basic fibroblast GF (bFGF) were also analyzed. The results showed that a significant reduction in ulcer area, restoration of skin structure, increase in collagen content, angiogenesis, and suppression of inflammation were possible with GF-cocktail G/O-NGD, indicating that G/O-NGD is a prospective carrier for local delivery of GF, improving wound healing processes.
治愈糖尿病足溃疡（DFUs）对许多糖尿病患者构成了严峻挑战。局部应用生物材料治疗DFUs最近受到了广泛关注。在此，我们展示了一种油包凝胶纳米凝胶分散体（G/O-NGD），它能够通过局部途径局部递送六种不同的生长因子（GFs），随后在小鼠体内进行评估。局部应用GF鸡尾酒G/O-NGD后，皮肤结构的宏观和微观变化均很明显，并测量了CD68和CD31水平以及胶原蛋白含量的变化。还分析了白细胞介素-6（IL-6）、转化生长因子β1（TGF-β1）和碱性成纤维细胞生长因子（bFGF）的表达和合成。结果表明，GF鸡尾酒G/O-NGD能够显著减少溃疡面积、恢复皮肤结构、增加胶原蛋白含量、促进血管生成并抑制炎症，这表明G/O-NGD是局部递送GF的一种有前景的载体，可改善伤口愈合过程。
