Li Ying, Wei Hongqi, Qian Mingtao
Department of Otolaryngology，Second Affiliated Hospital of Suzhou University，Suzhou，215004，China.
Department of Otolaryngology，Zhangjiagang First People's Hospital.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2024 Dec;38(12):1144-1148. doi: 10.13201/j.issn.2096-7993.2024.12.010.
To analyze the correlation between nasal mucosal microbiota diversity and the pathogenesis and prognosis of chronic sinusitis. A total of 80 patients with chronic sinusitis（CRS） admitted to Second Affiliated Hospital of Suzhou University were selected as the research group, and 80 patients with chronic dacryocystitis and nasal septum deviation without sinus inflammation admitted to our hospital during the same period were selected as the control group, nasal secretory specimens were collected under nasal endoscopic guidance by nasal swab, and matrix-assisted laser desorption ionization classification time mass spectrometry, anaerobic bacterial culture and common bacterial culture were performed to compare the differences in nasal mucosal flora between groups. Patients with chronic sinusitis were followed up for 6 months, and nasal secretions were collected again to detect microflora, and the patients were divided into relapse group（21 cases） and non-recurrence group（59 cases） according to whether the patients had relapse. Compare the diversity of nasal microbiota between groups. There were no significant differences in mean relative abundance（MRA） between the two groups of preoperative phylum Microbacterium verrucobacterium, Cyanobacterium phylum, Phylum Laubia mlaus, Porphyromonas species, Enterococcus species, Fusobacterium species, Enterobacter species, Enterobacter species, Erythrobacterium species, Ralstonia species, Bacteroides and Streptococcus species（>0.05）. The MRA of Acidobacterium, Proteobacteria, Actinomycetes MRA, Moraxia, Cyanobacterium, Corynebacterium and Staphylococcus were significantly lower than those of the control group（<0.05）, and the MRA of Escher-Shigella species, Fusobacterium, Bacteroides, Firmicutes, Neisseria, Pseudomonas, Haemophilus and Lactobacillus was significantly higher than that of the control group（<0.05）, and there was no significant difference in MRA at the level of nasal flora and genus before and after surgery in the relapsed group>0.05）. The MRA of Bacteroides after surgery was significantly lower in the non-recurrent group than that before surgery（<0.05）, the MRA of Corynebacterium and Actinomycetes was higher significantly than that of preoperative（<0.05）, and there was no significant difference in MRA of other species and phylum（>0.05）. The onset of CRS is related to nasal mucosal dysbacteria, and whether the dysbacteriosis improves after surgery is correlated with the prognosis of patients.
分析鼻黏膜微生物群多样性与慢性鼻窦炎发病机制及预后之间的相关性。选取苏州大学附属第二医院收治的80例慢性鼻窦炎（CRS）患者作为研究组，选取同期我院收治的80例慢性泪囊炎且鼻中隔偏曲但无鼻窦炎症的患者作为对照组，在鼻内镜引导下用鼻拭子采集鼻分泌物标本，进行基质辅助激光解吸电离分型时间质谱分析、厌氧菌培养及普通细菌培养，比较两组鼻黏膜菌群差异。对慢性鼻窦炎患者随访6个月，再次采集鼻分泌物检测微生物群，根据患者是否复发将患者分为复发组（21例）和未复发组（59例）。比较各组鼻微生物群的多样性。两组术前微小杆菌门、蓝细菌门、劳比亚菌门、卟啉单胞菌属、肠球菌属、梭杆菌属、肠杆菌属、红杆菌属、罗尔斯顿菌属、拟杆菌属和链球菌属的平均相对丰度（MRA）差异无统计学意义（>0.05）。嗜酸菌属、变形菌门、放线菌MRA、莫拉菌属、蓝细菌门、棒状杆菌属和葡萄球菌属的MRA显著低于对照组（<0.05），埃希-志贺菌属、梭杆菌属、拟杆菌属、厚壁菌门、奈瑟菌属、假单胞菌属、嗜血杆菌属和乳杆菌属的MRA显著高于对照组（<0.05），复发组手术前后鼻菌群和属水平的MRA差异无统计学意义（>0.05）。未复发组术后拟杆菌属的MRA显著低于术前（<0.05），棒状杆菌属和放线菌的MRA显著高于术前（<0.05），其他菌属和菌门的MRA差异无统计学意义（>0.05）。CRS的发病与鼻黏膜菌群失调有关，术后菌群失调是否改善与患者预后相关。
