Department of Otorhinolaryngology-Head and Neck Surgery, Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
Department of Otorhinolaryngology, First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Guangzhou, China.
Front Cell Infect Microbiol. 2022 Feb 17;12:786481. doi: 10.3389/fcimb.2022.786481. eCollection 2022.
Age-related changes in nasal bacterial microbiota of patients with chronic rhinosinusitis (CRS) remains unclear. In this study, we aimed to identify distinct characteristics of nasal bacterial microbiota between aged and younger patients with CRS through 16S rDNA gene sequencing. Patients with CRS undergoing endoscopic sinus surgery were recruited and separated into aged (≥60 years, median age = 66 years, N = 17) and younger (<60 years, median age = 35.5 years, N = 14) patients. Diversity, bacterial composition and metabolic activities of nasal microbiota between aged and younger patients were compared. Results have shown that levels of OTUs (p = 0.0173) and microbiota diversity (all p < 0.05) decreased significantly in aged patients. The abundance of phylum , and genus were significantly higher in aged patients, while the abundance of phylum , , and genus , were significantly higher in younger patients. In addition, predicted functional profiles have revealed that 41 KEGG pathways involving in 12 metabolic pathways, 4 genetic information processing, 3 environmental information processing, 4 cellular processes, 8 organismal systems, 6 human diseases, and 4 unclassified pathways were identified. Among which, the vast majority of metabolic activities are involved in replication and repair, membrane transport, translation, and the metabolism of amino acid, carbohydrate, energy, cofactors and vitamins, and nucleotide. On the level of the thirdly bacterial metabolic pathways, purine metabolism, glycine, serine and threonine metabolism, valine, leucine and isoleucine biosynthesis, glycolysis/gluconeogenesis and phenylalanine, tyrosine and tryptophan biosynthesis are significantly up-regulated while carbon fixation pathways in prokaryotesand methane metabolism are significantly down-regulated in aged patients. Overall, our analysis revealed that age-related physiological and pathological changes on the nasal mucosal surface may alter the host immune response and be highly associated with the nasal bacterial microbiota of patients with CRS. However, future studies are needed to elucidate the causal relationship.
年龄相关性变化在慢性鼻-鼻窦炎（CRS）患者的鼻腔细菌微生物群仍不清楚。在这项研究中，我们旨在通过 16S rDNA 基因测序来确定 CRS 老年和年轻患者鼻腔细菌微生物群之间的不同特征。招募接受内镜鼻窦手术的 CRS 患者，并将其分为老年（≥60 岁，中位年龄=66 岁，N=17）和年轻（<60 岁，中位年龄=35.5 岁，N=14）患者。比较老年和年轻患者之间鼻腔微生物群的多样性、细菌组成和代谢活性。结果表明，老年患者的 OTUs 水平（p=0.0173）和微生物多样性（所有 p<0.05）显著降低。厚壁菌门和属的丰度在老年患者中显著升高，而拟杆菌门、变形菌门、放线菌门和属、奈瑟菌属、嗜血杆菌属在年轻患者中显著升高。此外，预测的功能谱表明，涉及 12 种代谢途径、4 种遗传信息处理、3 种环境信息处理、4 种细胞过程、8 种生物体系统、6 种人类疾病和 4 种未分类途径的 41 种 KEGG 途径被识别。其中，绝大多数代谢活动涉及复制和修复、膜转运、翻译以及氨基酸、碳水化合物、能量、辅因子和维生素以及核苷酸的代谢。在第三级细菌代谢途径水平上，嘌呤代谢、甘氨酸、丝氨酸和苏氨酸代谢、缬氨酸、亮氨酸和异亮氨酸生物合成、糖酵解/糖异生和苯丙氨酸、酪氨酸和色氨酸生物合成在老年患者中显著上调，而原核生物的碳固定途径和甲烷代谢在老年患者中显著下调。总体而言，我们的分析表明，鼻腔黏膜表面的年龄相关生理和病理变化可能改变宿主免疫反应，并与 CRS 患者的鼻腔细菌微生物群高度相关。然而，需要进一步的研究来阐明因果关系。
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