Aruwa Joshua Ojodale, Bisong Sunday Agba, Obeten Kebe, Etukudo Ekom Monday, Timothy Neeza, Kureh Theophilus Gbednet, Okoruwa Godwin Aiyabalu, Pius Theophilus, Usman Ibe Michael
Department of Pharmacology and Toxicology, Kampala International University, Bushenyi, Uganda.
Department of Physiology, University of Calabar, Calabar, Nigeria.
J Exp Pharmacol. 2024 Nov 23;16:441-453. doi: 10.2147/JEP.S476773. eCollection 2024.
Fluoxetine (FLX) is a Selective Serotonin Re-uptake Inhibitor (SSRI) commonly used as a first-line treatment for depression, anxiety, and mood disorders. It can cause infertility in the male reproductive system through the release of Reactive Oxygen Species (ROS). This study aimed to evaluate the testiculo-protective potential of ascorbic acid against fluoxetine-induced spermatotoxicity in male Wistar rats.
This study assessed Vitamin C's effect on male fertility in fluoxetine-treated Wistar rats. Thirty rats (130 ± 40 g) were divided into six groups (n=5): Control (distilled water), fluoxetine 20 mg/kg, Vitamin C 100 mg/kg, fluoxetine 20 mg/kg + Vitamin C 50 mg/kg, fluoxetine 20 mg/kg + Vitamin C 100 mg/kg, and fluoxetine 20 mg/kg + Vitamin C 150 mg/kg. Treatments were administered daily via oral gavage for 60 days, followed by assessments of testicular weight, semen analysis, oxidative stress biomarkers (CAT and GPx), and histomorphology. The data was analyzed using one-way ANOVA and Turkey's post-hoc multiple comparison test, reporting as mean±SEM using The GraphPad Prism version 6.0 for Windows, with significance set at p<0.05.
Vitamin C, administered particularly at higher doses, significantly increased body weight, testicular weight, and antioxidant enzyme levels (glutathione peroxidase and catalase) while improving fertility parameters such as sperm count, motility, and viability in treated rats (P<0.05). Fluoxetine alone led to a significant reduction (P<0.05) in these parameters, but the combination with Vitamin C mitigated these effects. Histological analysis showed improved testicular structure in Vitamin C-treated groups, highlighting its protective role against fluoxetine-induced testicular damage.
Ascorbic acid has testiculoprotective potential in fluoxetine-induced spermatotoxicity, mainly owing to its antioxidant properties.
氟西汀（FLX）是一种选择性5-羟色胺再摄取抑制剂（SSRI），常用于抑郁症、焦虑症和情绪障碍的一线治疗。它可通过活性氧（ROS）的释放导致男性生殖系统不育。本研究旨在评估抗坏血酸对氟西汀诱导的雄性Wistar大鼠精子毒性的睾丸保护潜力。
本研究评估了维生素C对氟西汀处理的Wistar大鼠雄性生育能力的影响。30只大鼠（130±40克）分为六组（n=5）：对照组（蒸馏水）、氟西汀20毫克/千克、维生素C 100毫克/千克、氟西汀20毫克/千克+维生素C 50毫克/千克、氟西汀20毫克/千克+维生素C 100毫克/千克、氟西汀20毫克/千克+维生素C 150毫克/千克。通过口服灌胃每日给药60天，随后评估睾丸重量、精液分析、氧化应激生物标志物（CAT和GPx）和组织形态学。使用单因素方差分析和土耳其事后多重比较检验分析数据，使用适用于Windows的GraphPad Prism 6.0版本以平均值±标准误报告，显著性设定为p<0.05）。
特别是高剂量给予的维生素C显著增加了体重、睾丸重量和抗氧化酶水平（谷胱甘肽过氧化物酶和过氧化氢酶），同时改善了处理大鼠的生育参数，如精子数量、活力和存活率（P<0.05）。单独使用氟西汀导致这些参数显著降低（P<0.05），但与维生素C联合使用可减轻这些影响。组织学分析显示维生素C处理组的睾丸结构得到改善，突出了其对氟西汀诱导的睾丸损伤的保护作用。
抗坏血酸对氟西汀诱导的精子毒性具有睾丸保护潜力，主要归因于其抗氧化特性。
