Zhang Zehao, Schaefer Chloe, Jiang Weirong, Lu Ziyu, Lee Jasper, Sziraki Andras, Abdulraouf Abdulraouf, Wick Brittney, Haeussler Maximilian, Li Zhuoyan, Molla Gesmira, Satija Rahul, Zhou Wei, Cao Junyue
Laboratory of Single-Cell Genomics and Population Dynamics, The Rockefeller University, New York, NY, USA.
The David Rockefeller Graduate Program in Bioscience, The Rockefeller University, New York, NY, USA.
Science. 2025 Jan 17;387(6731):eadn3949. doi: 10.1126/science.adn3949.
To elucidate aging-associated cellular population dynamics, we present PanSci, a single-cell transcriptome atlas profiling >20 million cells from 623 mouse tissues across different life stages, sexes, and genotypes. This comprehensive dataset reveals >3000 different cellular states and >200 aging-associated cell populations. Our panoramic analysis uncovered organ-, lineage-, and sex-specific shifts in cellular dynamics during life-span progression. Moreover, we identify both systematic and organ-specific alterations in immune cell populations associated with aging. We further explored the regulatory roles of the immune system on aging and pinpointed specific age-related cell population expansions that are lymphocyte dependent. Our "cell-omics" strategy enhances comprehension of cellular aging and lays the groundwork for exploring the complex cellular regulatory networks in aging and aging-associated diseases.
为了阐明与衰老相关的细胞群体动态，我们展示了PanSci，这是一个单细胞转录组图谱，对来自不同生命阶段、性别和基因型的623个小鼠组织中的超过2000万个细胞进行了分析。这个全面的数据集揭示了超过3000种不同的细胞状态和超过200个与衰老相关的细胞群体。我们的全景分析揭示了生命周期进程中细胞动态在器官、谱系和性别方面的特异性变化。此外，我们确定了与衰老相关的免疫细胞群体中的系统性和器官特异性改变。我们进一步探索了免疫系统对衰老的调节作用，并确定了特定的与年龄相关的细胞群体扩张，这些扩张依赖于淋巴细胞。我们的“细胞组学”策略增强了对细胞衰老的理解，并为探索衰老及衰老相关疾病中的复杂细胞调节网络奠定了基础。
