Department of Biosciences, Università Degli Studi Di Milano, Via Celoria 26, 20133, Milan, Italy.
Department of Agricultural and Environmental Sciences, Università Degli Studi Di Milano, Via Celoria 2, 20133, Milan, Italy.
Plant Reprod. 2024 Nov 29;38(1):4. doi: 10.1007/s00497-024-00514-w.
We describe transcriptional dynamics at the rice shoot apex by integrating time resolved single nuclei RNA-seq with bulk RNA-seq data. In rice, short days trigger floral transition and the transcriptional reprogramming of the shoot apex to become reproductive. We integrated time-resolved bulk RNA-seq with single nuclei RNA-seq analysis to gain a refined understanding of the transcriptional programs induced at the shoot apex during floral transition. Our analyses highlighted technological and conceptual differences between single nuclei RNA-seq and bulk RNA-seq data and described previously uncharacterized transcriptional programs associated with the early steps of floral induction in rice.
我们通过整合时间分辨的单个细胞核 RNA-seq 与整体 RNA-seq 数据，描述了水稻芽尖的转录动态。在水稻中，短日照会引发花转变，从而使芽尖的转录重编程成为生殖状态。我们将时间分辨的整体 RNA-seq 与单个细胞核 RNA-seq 分析相结合，以更深入地了解花转变过程中在芽尖诱导的转录程序。我们的分析突出了单个细胞核 RNA-seq 和整体 RNA-seq 数据之间在技术和概念上的差异，并描述了与水稻花诱导早期步骤相关的以前未被描述的转录程序。
