Tipping William J, Faulds Karen, Graham Duncan
Pure and Applied Chemistry, University of Strathclyde, Technology and Innovation Centre, 99 George Street, Glasgow, G1 1RD, United Kingdom.
Chem Biomed Imaging. 2024 Oct 4;2(11):733-743. doi: 10.1021/cbmi.4c00057. eCollection 2024 Nov 25.
Super-resolution optical imaging overcomes the diffraction limit in light microscopy to enable the visualization of previously invisible molecular details within a sample. The realization of super-resolution imaging based on stimulated Raman scattering (SRS) microscopy represents a recent area of fruitful development that has been used to visualize cellular structures in three dimensions, with multiple spectroscopic colors at the nanometer scale. Several fundamental approaches to achieving super-resolution SRS imaging have been reported, including optical engineering strategies, expansion microscopy, deconvolution image analysis, and photoswitchable SRS reporters as methods to break the diffraction limit. These approaches have enabled the visualization of biological structures, cellular interactions, and dynamics with unprecedented detail. In this Perspective, an overview of the current strategies and capabilities for achieving super-resolution SRS imaging will be highlighted together with an outlook on potential directions of this rapidly evolving field.
超分辨率光学成像克服了光学显微镜中的衍射极限，能够可视化样本中以前不可见的分子细节。基于受激拉曼散射（SRS）显微镜的超分辨率成像的实现是一个近期成果丰硕的发展领域，已被用于在纳米尺度上以多种光谱颜色对细胞结构进行三维可视化。已经报道了几种实现超分辨率SRS成像的基本方法，包括光学工程策略、扩展显微镜、去卷积图像分析以及可光开关的SRS报告分子作为突破衍射极限的方法。这些方法使人们能够以前所未有的细节可视化生物结构、细胞相互作用和动态过程。在这篇综述中，将重点介绍当前实现超分辨率SRS成像的策略和能力，并展望这个快速发展领域的潜在方向。
