Qu Xian, Ji Yahui, Long Jieyi, Zheng Donglin, Qiao Zhonghui, Lin Yue, Lu Chang, Zhou Yifa, Cheng Hairong
Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, China.
Infinitus (China) Company Ltd., Guangzhou 510623, China.
Food Chem. 2025 Feb 28;466:142209. doi: 10.1016/j.foodchem.2024.142209. Epub 2024 Nov 23.
The β-1,6-glucans from Lentinus edodes have a variety of biological activities. However, the research on extraction and separation of β-1,6-glucans from L. edodes is limited and the yield is low. In the present study, we obtained the high-yield and -purity β-1,6-glucans (ALEPA) from L. edodes by using a sequential extraction and separation process, which is simple and suitable for industrialization. ALEPA significantly promoted the proliferation of splenic T lymphocytes and enhanced the phagocytosis activity of peritoneal macrophages in vivo. 16S rRNA sequencing results showed that ALEPA significantly increased the α diversity of gut microbiota and upregulated the relative abundances of short chain fatty acids (SCFAs)-producing bacterial species. Consistently, the SCFAs in the cecum of mice were upregulated. On a mechanical level, we found that the immunomodulatory effect of ALEPA depended on gut microbiota. Collectively, ALEPA is a promising functional food ingredient that regulates gut microbiota and enhances immune function.
香菇中的β-1,6-葡聚糖具有多种生物活性。然而，从香菇中提取和分离β-1,6-葡聚糖的研究有限，产量较低。在本研究中，我们采用一种简单且适合工业化的顺序提取和分离工艺，从香菇中获得了高产率和高纯度的β-1,6-葡聚糖（ALEPA）。ALEPA显著促进了脾脏T淋巴细胞的增殖，并增强了体内腹腔巨噬细胞的吞噬活性。16S rRNA测序结果表明，ALEPA显著增加了肠道微生物群的α多样性，并上调了产生短链脂肪酸（SCFAs）的细菌种类的相对丰度。一致地，小鼠盲肠中的SCFAs也上调了。在机制层面上，我们发现ALEPA的免疫调节作用依赖于肠道微生物群。总体而言，ALEPA是一种有前景的功能性食品成分，可调节肠道微生物群并增强免疫功能。
