Faculty of Engineering and Natural Sciences, Tampere University, Hervanta Campus, PO Box 527, Tampere, FI-33014, Finland.
Sci Rep. 2024 Nov 29;14(1):29678. doi: 10.1038/s41598-024-81124-4.
The efficient utilization of lignocellulosic hydrolysates in bioprocesses is impeded by their complex composition and the presence of toxic compounds, such as furan aldehydes, formed during lignocellulose pretreatment. Biological detoxification of these furan aldehydes offers a promising solution to enhance the utilization of lignocellulosic hydrolysates. Acinetobacter baylyi ADP1 is known to metabolize furan aldehydes, yet the complete spectrum of reaction products and dynamics remains unclear. Here, we determined the detoxification metabolites of furfural and 5-hydroxymethylfurfural in A. baylyi ADP1 and studied the kinetics of detoxification. The results indicate that detoxification in A. baylyi ADP1 follows a typical alcohol-aldehyde-acid scheme, with furoic acid and 5-hydroxymethyl-2-furancarboxylic acid as the final products for furfural and 5-hydroxymethylfurfural, respectively. Both end products were found to be less toxic for cells than their unmodified forms. These findings underscore the potential of A. baylyi ADP1 in detoxifying lignocellulosic hydrolysates for bioprocess applications.
木质纤维素水解物的有效利用受到其复杂成分和有毒化合物（如在木质纤维素预处理过程中形成的糠醛醛）的阻碍。这些糠醛醛的生物解毒为提高木质纤维素水解物的利用提供了一个有前途的解决方案。众所周知，不动杆菌 ADP1 可以代谢糠醛醛，但反应产物和动力学的完整范围尚不清楚。在这里，我们确定了糠醛和 5-羟甲基糠醛在 A. baylyi ADP1 中的解毒代谢物，并研究了解毒动力学。结果表明，A. baylyi ADP1 中的解毒作用遵循典型的醇-醛-酸方案，糠醛的最终产物为糠酸，5-羟甲基-2-糠酸为 5-羟甲基糠醛的最终产物。发现这两种终产物的毒性都比其未修饰形式低。这些发现强调了 A. baylyi ADP1 在用于生物过程应用的木质纤维素水解物解毒方面的潜力。
