Hong Le, Li Wen, Qi Manlin, Dong Weinan, Liu Chengyu, Li Meiqi, Zhong Yuan, Wu Zhennan, Li Chunyan, Bai Xue, Wang Lin
Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, China.
State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University Changchun, Changchun to 130012, China.
Nano Lett. 2024 Dec 11;24(49):15547-15556. doi: 10.1021/acs.nanolett.4c03503. Epub 2024 Nov 30.
Antimicrobial sonodynamic therapy (SDT) holds great promise in clinical practice regarding its noninvasiveness, high safety profile, and absence of resistance concern. However, exploring high-efficiency sonodynamic sensitizers is slow-moving and remains a big challenge. We, herein, employed gold nanoclusters (Au NCs) as a novel class of sonodynamic sensitizers, demonstrating notable antimicrobial efficacy in treating infected wounds. Specifically, l-arginine (Arg) and 6-azido-2-thiothymidine (ATT) cocapped Au NCs featured enhanced structural rigidity, suppressing nonradiative relaxation of excited electrons and achieving a reactive oxygen species (ROS) yield exceeding 45%. Moreover, the modification of ATT-Au NCs by Arg imparted amino acid-like properties to the Au NCs, while the ultrasound (US) up-regulates the expression of porins in . This synergy resulted in a burst of ROS production within the bacterial cells, ultimately leading to a four-order-of-magnitude reduction in microbial viability.
抗菌声动力疗法（SDT）因其非侵入性、高安全性以及不存在耐药性问题，在临床实践中具有广阔前景。然而，探索高效的声动力敏化剂进展缓慢，仍然是一个巨大的挑战。在此，我们采用金纳米团簇（Au NCs）作为一类新型的声动力敏化剂，在治疗感染伤口方面展现出显著的抗菌效果。具体而言，L-精氨酸（Arg）和6-叠氮基-2-硫代胸苷（ATT）共包覆的Au NCs具有增强的结构刚性，抑制了激发电子的非辐射弛豫，并实现了超过45%的活性氧（ROS）产率。此外，Arg对ATT-Au NCs的修饰赋予了Au NCs类似氨基酸的性质，而超声（US）上调了细菌中外膜孔蛋白的表达。这种协同作用导致细菌细胞内产生大量ROS，最终使微生物活力降低四个数量级。
