Huang Xueying, Wang Yu, Li Feng, Zhao Hui, Zeng Liwang, Li Huiliang, Gu Fenglin, Tan Deguan, Hu Wei, Guo Anping, Ji Changmian, He Linwen
School of Marine Biology and Fisheries, Hainan University, Haikou 570228, China; National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Sanya 572025, China.
National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Sanya 572025, China; Sanya Nanfan Research Institution of Hainan University, Sanya 572025, China.
Int J Biol Macromol. 2025 Jan;285:138193. doi: 10.1016/j.ijbiomac.2024.138193. Epub 2024 Nov 29.
Freshwater soaking of the conchocelis is often used to reduce yellow spot, white spot, and mud red disease in Pyropia yezoensis. However, the understanding of physiological, transcriptomic, and metabolomic changes for the conchocelis under freshwater stress remains limited. Here, we comprehensively explored the dynamic changes of physiological activities, transcriptomes, and metabolomes of the conchocelis under three points of freshwater stress (0 h, 4 h, and 24 h) and one point of seawater recovery (R2h). We found that the content of photosynthetic pigments, soluble proteins, and photosynthesis performance significantly responded to freshwater stress. Metabolomic analysis identified a total of 24 metabolites, including 15 DAMs, suggesting the metabolites changes in the conchocelis in response to freshwater stress. Additionally, comparative transcriptome and metabolome analyses identified a black co-expression module that was strongly correlated with the DAMs. Furthermore, this module was predominantly enriched in carbohydrate and amino acid metabolism pathways. We found that PyDLD, PyPGK, and PyBCKDHA were key genes in hub-networks, which are potentially involved in changes of leucine, valine, isoleucine, lactate, and floridoside during freshwater stress. These findings reveal the genetic basis of the dynamic changes of physiological activities, transcriptome, and metabolome in the Py. yezoensis conchocelis during freshwater soaking for disease control.
紫菜丝状体的淡水浸泡常用于减少条斑紫菜的黄斑、白斑和泥红病。然而，对于淡水胁迫下紫菜丝状体的生理、转录组和代谢组变化的了解仍然有限。在此，我们全面探究了在三个淡水胁迫时间点（0小时、4小时和24小时）以及一个海水恢复时间点（R2小时）下紫菜丝状体的生理活动、转录组和代谢组的动态变化。我们发现光合色素、可溶性蛋白的含量以及光合作用性能对淡水胁迫有显著响应。代谢组分析共鉴定出24种代谢物，包括15种差异积累代谢物，表明紫菜丝状体中的代谢物因淡水胁迫而发生变化。此外，比较转录组和代谢组分析确定了一个与差异积累代谢物高度相关的黑色共表达模块。此外，该模块主要富集于碳水化合物和氨基酸代谢途径。我们发现PyDLD、PyPGK和PyBCKDHA是枢纽网络中的关键基因，它们可能参与了淡水胁迫期间亮氨酸、缬氨酸、异亮氨酸、乳酸和 floridoside的变化。这些发现揭示了条斑紫菜丝状体在淡水浸泡防病过程中生理活动、转录组和代谢组动态变化的遗传基础。
