State Key Laboratory of Metastable Materials Science & Technology and Key Laboratory for Microstructural Material Physics of Hebei Province, School of Science, Yanshan University, Qinhuangdao, 066004, PR China.
State Key Laboratory of Metastable Materials Science & Technology and Key Laboratory for Microstructural Material Physics of Hebei Province, School of Science, Yanshan University, Qinhuangdao, 066004, PR China.
Anal Chim Acta. 2025 Jan 2;1333:343385. doi: 10.1016/j.aca.2024.343385. Epub 2024 Nov 1.
Surface plasmon resonance (SPR) sensing technology has been widely used in biometrics, but the weak detection capability and low sensitivity limit the development of SPR biosensors. In this work, we propose to employ the transition metal disulfide (TMD) material MoS to induce the SPR effect into the near-infrared band. The aim of this work is to develop a near-infrared sensor capable of quantitatively detecting the concentration of cDNA, which is able to solve the problems of low sensitivity, parameter crosstalk and so on.
The results show that the sensitivity of the SPR sensor at infrared wavelengths is 1.69 times higher than that of visible, and the infrared wave is more suitable as an excitation source for the SPR effect because it has a stronger evanescent field. In addition, we have prepared a DNA hybridization sensor that can work in the near-infrared band to detect complementary DNA (cDNA) concentration. Moreover, a combined cascade and parallel strategy realized the temperature and PH detection. The sensor uses the photonic crystal fiber as the platform, silver film as an excitation layer, and MoS as a modulation layer. The optimal parameters of the sensor were determined during the experiment, and the three-parameter detection was successfully realized. The experimental results show that the three sensing channels can work independently, and the DNA hybridization probe can achieve selective detection of cDNA with a sensitivity as high as 0.22 nm/(nmol/L).
The proposed three-channel DNA hybridization sensor has high sensitivity and accuracy. The innovation of this work is to demonstrate that infrared waves are more suitable to be used as the excitation source of SPR sensors and that the proposed three-channel sensor has a promising future in early diagnosis and biosensing.
表面等离子体共振（SPR）传感技术已广泛应用于生物识别领域，但微弱的检测能力和低灵敏度限制了 SPR 生物传感器的发展。在这项工作中，我们提出利用过渡金属二硫化物（TMD）材料 MoS 将 SPR 效应诱导到近红外波段。本工作的目的是开发一种能够定量检测 cDNA 浓度的近红外传感器，以解决灵敏度低、参数串扰等问题。
结果表明，近红外波长下 SPR 传感器的灵敏度比可见光提高了 1.69 倍，且红外光作为 SPR 效应的激发源更合适，因为它具有更强的消逝场。此外，我们制备了一种可在近红外波段工作的 DNA 杂交传感器，用于检测互补 DNA（cDNA）浓度。此外，采用级联和并行组合策略实现了温度和 PH 值检测。该传感器以光子晶体光纤为平台，银膜为激励层，MoS 为调制层。实验确定了传感器的最佳参数，并成功实现了三参数检测。实验结果表明，三个传感通道可以独立工作，DNA 杂交探针能够实现对 cDNA 的选择性检测，灵敏度高达 0.22nm/（nmol/L）。
所提出的三通道 DNA 杂交传感器具有高灵敏度和准确性。这项工作的创新之处在于证明了红外光更适合作为 SPR 传感器的激发源，并且所提出的三通道传感器在早期诊断和生物传感方面具有广阔的应用前景。
