Tene Talia, Bellucci Stefano, Vacacela Gomez Cristian
Department of Chemistry, Universidad Técnica Particular de Loja, Loja 110160, Ecuador.
INFN-Laboratori Nazionali di Frascati, Via E. Fermi 54, 00044 Frascati, Italy.
Biosensors (Basel). 2025 Jan 4;15(1):21. doi: 10.3390/bios15010021.
The COVID-19 pandemic has highlighted the urgent need for rapid, sensitive, and reliable diagnostic tools for detecting SARS-CoV-2. In this study, we developed and optimized a surface plasmon resonance (SPR) biosensor incorporating advanced materials to enhance its sensitivity and specificity. Key parameters, including the thickness of the silver layer, silicon nitride dielectric layer, molybdenum disulfide (MoS) layers, and ssDNA recognition layer, were systematically optimized to achieve the best balance between sensitivity, resolution, and attenuation. The optimized configuration, consisting of a 45 nm silver layer, a 13 nm silicon nitride layer, 2 MoS layers, and a 5 nm ssDNA layer, demonstrated superior performance for detecting SARS-CoV-2 in PBS solution. The biosensor exhibited high sensitivity at low viral concentrations, achieving a sensitivity of 375.01°/RIU, a detection accuracy of 0.002, and a quality factor of 38.34 at 1.0 mM SARS-CoV-2 concentration. Performance metrics validated the sensor's capability for reliable detection, particularly in early-stage diagnostics where timely intervention is critical. Moreover, the biosensor's linear response to refractive index changes confirmed its potential for quantitative viral concentration analysis. This study underlines the significance of integrating advanced materials, such as MoS and silicon nitride, to enhance SPR biosensor performance. The findings establish the proposed biosensor as a robust and precise diagnostic tool for SARS-CoV-2 detection, with potential applications in clinical diagnostics and epidemiological monitoring.
新冠疫情凸显了对用于检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的快速、灵敏且可靠的诊断工具的迫切需求。在本研究中，我们开发并优化了一种表面等离子体共振（SPR）生物传感器，该传感器结合了先进材料以提高其灵敏度和特异性。包括银层、氮化硅介电层、二硫化钼（MoS）层和单链DNA识别层厚度在内的关键参数被系统地优化，以在灵敏度、分辨率和衰减之间实现最佳平衡。由45纳米银层、13纳米氮化硅层、2个MoS层和5纳米单链DNA层组成的优化配置，在磷酸盐缓冲盐水（PBS）溶液中检测SARS-CoV-2时表现出卓越性能。该生物传感器在低病毒浓度下具有高灵敏度，在1.0毫摩尔SARS-CoV-2浓度时，灵敏度达到375.01°/RIU，检测精度为0.002，品质因数为38.34。性能指标验证了该传感器进行可靠检测的能力，特别是在早期诊断中，及时干预至关重要。此外，该生物传感器对折射率变化的线性响应证实了其在定量病毒浓度分析方面的潜力。本研究强调了整合诸如MoS和氮化硅等先进材料以提高SPR生物传感器性能的重要性。这些发现确立了所提出的生物传感器作为一种用于SARS-CoV-2检测的强大且精确的诊断工具，在临床诊断和流行病学监测中具有潜在应用。
