Selim Samy, Almuhayawi Mohammed S, Alruhaili Mohammed H, Tarabulsi Muyassar K, Saddiq Amna A, Elamir Mohammed Yagoub Mohammed, Amin Mohamed A, Al Jaouni Soad K
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, 72388, Sakaka, Saudi Arabia.
Department of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.
Sci Rep. 2025 Feb 12;15(1):5263. doi: 10.1038/s41598-025-87881-0.
The uniqueness and novelty of this study lies in the ability of Mentha longifolia leaves extract (MLLE) to synthesize bimetallic NPs (NPs) of zinc oxide and magnesium oxide as nanocomposite (ZnO/MgO NPs) for the first time. Medicinal plants extracts are a more environmentally friendly method of creating NPs than physical or chemical methods. The specific objectives of the research were employed this nanocomposite compared to plant extract as antibacterial, anti-diabetic, antioxidant agents. Also, the possibility of using this nanocomposite as plant stimulator for reducing saline water stress on economic plants to cope with the scarcity of freshwater in the agricultural sector. In comparison to nanocomposite, MLLE exhibited high inhibition zones 28 ± 0.1, 26 ± 0.2, 26 ± 0.1, 25 ± 0.2, 25 ± 0.1 and 24 ± 0.1 mm in medium inoculated by E. faecalis, E. coli, S. typh, M. circinelloid C. albicans, and S. aureus, respectively. It was shown from the DPPH data that ZnO/MgO NPs' IC value (52.55 ± 0.98 µg/mL) was lower than the extract's (299.27 ± 1.59 µg/mL) when compared to ascorbic (195.15 ± 1.63 µg/mL). Compared to acarbose, ZnO/MgO NPs exhibited superior activity against α-Amylase inhibition percentage, as evidenced by their IC value of 117.02 ± 0.56 µg/mL. In contrast to ZnO/MgO NPs, acarbose had a lower IC value of 22.15 ± 0.76 µg/mL. ZnO/MgO NPs were added to the soil cultivated by cucumber plants (A pots experiment) at quantities of 0, 200, and 400 mg/kg. Bimetallic ZnO/MgO NPs, particularly at 200 ppm, improved the shoot and root lengths and fresh weight of shoot, but they also seemed to reduce the level stress indicator of MDA, HO, and antioxidant enzymes (peroxidase and polyphenol oxidase). As a result, ZnO/MgO NPs may be employed as a unique approach to boost plant growth under salinity stress.
本研究的独特性和新颖之处在于，长叶薄荷叶片提取物（MLLE）首次具备合成氧化锌和氧化镁双金属纳米颗粒（NPs）作为纳米复合材料（ZnO/MgO NPs）的能力。与物理或化学方法相比，药用植物提取物是一种更环保的制备纳米颗粒的方法。本研究的具体目标是，将这种纳米复合材料作为抗菌、抗糖尿病、抗氧化剂与植物提取物进行比较。此外，探讨了使用这种纳米复合材料作为植物刺激剂以减轻经济作物盐水胁迫的可能性，从而应对农业部门淡水资源短缺的问题。与纳米复合材料相比，在接种粪肠球菌、大肠杆菌、伤寒沙门氏菌、环状念珠菌、白色念珠菌和金黄色葡萄球菌的培养基中，MLLE分别表现出28±0.1、26±0.2、26±0.1、25±0.2、25±0.1和24±0.1毫米的高抑菌圈。从DPPH数据可知，与抗坏血酸（195.15±1.63微克/毫升）相比，ZnO/MgO NPs的IC值（52.55±0.98微克/毫升）低于提取物的IC值（299.27±1.59微克/毫升）。与阿卡波糖相比，ZnO/MgO NPs对α -淀粉酶抑制率表现出更强的活性，其IC值为117.02±0.56微克/毫升可证明这一点。相比之下，阿卡波糖的IC值较低，为22.15±0.76微克/毫升。在黄瓜植株种植的土壤中添加ZnO/MgO NPs（盆栽实验），添加量分别为0、200和400毫克/千克。双金属ZnO/MgO NPs，尤其是在200 ppm时，可提高地上部和根部长度以及地上部鲜重，但似乎也降低了丙二醛、过氧化氢和抗氧化酶（过氧化物酶和多酚氧化酶）的胁迫指标水平。因此，ZnO/MgO NPs可作为一种独特的方法来促进盐胁迫下的植物生长。
