Donohoe M J, Rush B F, Machiedo G W, Barillo D J, Murphy T F
Surg Gynecol Obstet. 1986 Apr;162(4):323-33.
It has been proposed that the isolated hepatocyte is an excellent model for the study of cellular changes during and after hemorrhagic shock. To investigate this proposition the biochemical and morphologic changes in the isolated hepatocyte of shock injured rats were documented. Use of the isolated hepatocyte allows direct measurement of intracellular biochemical changes which we find corroborates the results of basic shock studies done on rats using liver slices, perfused liver or specimens taken at biopsy. Morphologic changes in the shocked liver of the rat are noted using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The results are correlated with the biochemical changes. SEM of the isolated hepatocyte reveals marked swelling immediately after shock with some scattered blebs. The cells show some recovery at two hours with near complete normality restored at 24 hours. TEM of tissue and isolated cells reveal vacuolization and mitochondrial changes in the early post shock period with return to normality at 24 hours after shock. The shock injured hepatocyte is a reasonably faithful representative of the events which have taken place in the liver from which it is isolated. It can be used as a model for treatment in vivo or as a device for comparison of the effects of different environments in vivo or in vitro.
有人提出，分离的肝细胞是研究失血性休克期间及之后细胞变化的理想模型。为了研究这一观点，记录了休克损伤大鼠分离肝细胞的生化和形态学变化。使用分离的肝细胞可以直接测量细胞内生化变化，我们发现这证实了使用肝切片、灌注肝脏或活检标本对大鼠进行的基础休克研究结果。使用透射电子显微镜（TEM）和扫描电子显微镜（SEM）观察大鼠休克肝脏的形态学变化。结果与生化变化相关。分离肝细胞的SEM显示休克后立即出现明显肿胀，有一些散在的小泡。细胞在两小时时显示出一些恢复，24小时时几乎完全恢复正常。组织和分离细胞的TEM显示休克后早期有空泡化和线粒体变化，休克后24小时恢复正常。休克损伤的肝细胞相当忠实地代表了其分离自的肝脏中发生的事件。它可用作体内治疗的模型，或用作比较体内或体外不同环境影响的工具。
