Rush B F, Redan J A, Flanagan J J, Heneghan J B, Hsieh J, Murphy T F, Smith S, Machiedo G W
Department of Surgery, New Jersey Medical School, Newark 07103.
Ann Surg. 1989 Sep;210(3):342-5; discussion 346-7. doi: 10.1097/00000658-198909000-00010.
We have recently reported the rapid appearance of bacteria and endotoxin in the blood of rats and of trauma patients in the course of 30 minutes to 2 hours of hemorrhagic shock. The current study was designed to determine the effect of this bacteremia and endotoxemia on survival. Thirty-three conventional (C:group 1) and 36 germ-free (GF:group 2) Sprague Dawley rats were subjected to our previously described model of treated hemorrhagic shock. Survival in the GF group was significantly better than the C group at 24, 48, and 72 hours after shock. Endotoxin levels were elevated in 88% of C group during shock and in 28% of GF group. The gut of the GF animal contains endotoxin (26 ng/gm of stool) as does the sterile food supply (393 ng/gm of rat chow).
我们最近报道，在失血性休克30分钟至2小时内，大鼠和创伤患者血液中会迅速出现细菌和内毒素。本研究旨在确定这种菌血症和内毒素血症对存活率的影响。33只常规（C组：第1组）和36只无菌（GF组：第2组）的Sprague Dawley大鼠接受了我们之前描述的治疗性失血性休克模型。休克后24、48和72小时，GF组的存活率显著高于C组。休克期间，C组88%的大鼠内毒素水平升高，GF组为28%。GF动物的肠道含有内毒素（每克粪便26纳克），无菌食物供应中也含有内毒素（每克大鼠饲料393纳克）。
