MRC- MRC Laboratory of Molecular Biology, Cambridge, UK.
Methods Mol Biol. 2025;2872:247-255. doi: 10.1007/978-1-0716-4224-5_17.
During asymmetric cell division, cortical polarity cues drive the polarization of the microtubule cytoskeleton to ensure the generation of two daughter cells with different fates. While this a critical process for development and tissue homeostasis, the underlying molecular mechanisms orchestrating those processes are not completely understood, especially in mammals. Here, we present an assay that allows the study of the molecular mechanisms driving mammalian asymmetric cell division in a high-throughput manner by capitalizing on protein design to engineer cortical polarity of virtually any protein of interest in otherwise unpolarized mammalian culture cells.
在不对称细胞分裂过程中，皮质极性线索驱动微管细胞骨架的极化，以确保产生具有不同命运的两个子细胞。虽然这是发育和组织内稳态的关键过程，但调节这些过程的潜在分子机制尚未完全了解，尤其是在哺乳动物中。在这里，我们提出了一种测定方法，通过利用蛋白质设计来工程化实际上任何感兴趣的蛋白质的皮质极性，从而在 otherwise unpolarized 哺乳动物培养细胞中以高通量的方式研究驱动哺乳动物不对称细胞分裂的分子机制。
