Equipe labellisée Fondation ARC, Aix Marseille University, CNRS, UMR 7288, IBDM, Turing Center for Living Systems, Marseille, France.
Methods Mol Biol. 2025;2872:257-267. doi: 10.1007/978-1-0716-4224-5_18.
Live imaging of microtubules (MTs) allows obtaining mechanistic insights into cell division. In literature, mitotic spindle dynamics have been investigated in mammalian systems largely focusing on established cell lines. Here, we describe a detailed protocol that investigates MT dynamics during cell division in a 3D mouse intestinal organoid model that more accurately captures the in vivo system.
活细胞成像微管（MTs）可深入了解细胞分裂的机制。在文献中，有大量关于哺乳动物系统有丝分裂纺锤体动力学的研究，主要集中在已建立的细胞系上。在这里，我们描述了一个详细的方案，用于研究 3D 小鼠肠类器官模型中细胞分裂过程中的 MT 动力学，该模型更准确地捕获了体内系统。
