He Junlin, Liu Yefei, Xu Hongzhen, Wei Xiaolin, Chen Meihua
Department of Periodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai, China.
Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China.
Clin Oral Investig. 2024 Dec 2;28(12):675. doi: 10.1007/s00784-024-06074-7.
To characterize the subgingival microbiota in subjects with stage I/II periodontitis (moderate periodontitis, MP), stage III/IV periodontitis (severe periodontitis, SP), and periodontal health (PH) at the same probing depth (PD) (shallow ≤ 3 mm, moderate 4-6 mm, or deep ≥ 7 mm), and to investigate the changes associated with probing depth progression.
100 subgingival plaque samples were collected from 50 subjects (16 MP, 17 SP and 17 PH), forming six groups: PHS (PH, shallow), MPS (MP, shallow), MPM (MP, moderate), SPS (SP, shallow), SPM (SP, moderate), and SPD (SP, deep). Samples were analyzed using high-throughput sequencing.
The subgingival microbiome showed significant differences associated with both PD and periodontitis stage (p < 0.05). With increasing PD, alpha diversity initially increased and then decreased. Pathogenic genera like Fusobacterium, Filifactor, and Porphyromonas increased, while health-associated genera like Streptococcus and Haemophilus decreased. At shallow sites, the PHS, MPS, and SPS groups showed similar community structure. At moderate and deep sites, the SPM and SPD groups exhibited significant differences in community structure compared to the MPM group, with the SPM and SPD groups showing decreased abundances of Actinomyces and increased abundances of Treponema. The microbial co-networks in the SPD and SPM groups exhibited greater complexity and connectivity and were more resilient to random microbial or node removal.
The subgingival microbiome shows strong associations with PD and periodontitis stage.
Once periodontitis progresses to stage III/IV, reconstructing a healthy subgingival microbiome may be challenging, emphasizing the importance of early prevention.
在相同探诊深度（PD）（浅≤3mm、中度4 - 6mm或深≥7mm）的情况下，对I/II期牙周炎（中度牙周炎，MP）、III/IV期牙周炎（重度牙周炎，SP）和牙周健康（PH）受试者的龈下微生物群进行特征分析，并研究与探诊深度进展相关的变化。
从50名受试者（16名MP、17名SP和17名PH）中收集100份龈下菌斑样本，形成六组：PHS（PH，浅）、MPS（MP，浅）、MPM（MP，中度）、SPS（SP，浅）、SPM（SP，中度）和SPD（SP，深）。使用高通量测序对样本进行分析。
龈下微生物群显示出与PD和牙周炎阶段均相关的显著差异（p < 0.05）。随着PD增加，α多样性最初增加然后降低。诸如梭杆菌属、丝状杆菌属和卟啉单胞菌属等致病属增加，而诸如链球菌属和嗜血杆菌属等与健康相关的属减少。在浅部位，PHS、MPS和SPS组显示出相似的群落结构。在中度和深部位，与MPM组相比，SPM和SPD组在群落结构上表现出显著差异，SPM和SPD组放线菌属丰度降低，密螺旋体属丰度增加。SPD和SPM组中的微生物共网络表现出更大的复杂性和连通性，并且对随机微生物或节点去除更具弹性。
龈下微生物群与PD和牙周炎阶段密切相关。
一旦牙周炎发展到III/IV期，重建健康的龈下微生物群可能具有挑战性，这强调了早期预防的重要性。
