Periodontology Unit, Centre for Host Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King's College London, London, UK.
Centre for Oral Immunobiology & Regenerative Medicine & Centre for Oral Clinical Research, Institute of Dentistry, Barts and The London School of Medicine and Dentistry, Queen Mary University London, London, UK.
J Clin Periodontol. 2020 Aug;47(8):980-990. doi: 10.1111/jcpe.13330. Epub 2020 Jun 26.
The primary aim of this investigation was to analyse the periodontal microbiome in patients with aggressive periodontitis (AgP) following treatment.
Sixty-six AgP patients were recalled on average 7 years after completion of active periodontal treatment and had subgingival plaque samples collected and processed for 16S rRNA gene sequencing analyses.
Of 66 participants, 52 showed persistent periodontal disease, while 13 participants were considered as "successfully treated AgP" (no probing pocket depths >4 mm) and 1 was fully edentulous. Genera associated with persistent generalized disease included Actinomyces, Alloprevotella, Capnocytophaga, Filifactor, Fretibacterium, Fusobacterium, Leptotrichia, Mogibacterium, Saccharibacteria [G-1], Selenomonas and Treponema. "Successfully treated" patients harboured higher proportions of Haemophilus, Rothia, and Lautropia and of Corynebacterium, Streptococcus and Peptidiphaga genera. Overall, patients with persistent generalized AgP (GAgP) revealed higher alpha diversity compared to persistent localized AgP (LAgP) and stable patients (p < .001). Beta diversity analyses revealed significant differences only between stable and persistent GAgP groups (p = .004).
Patients with persistent AgP showed a more dysbiotic subgingival biofilm than those who have been successfully treated. It remains to be established whether such differences were predisposing to disease activity or were a result of a dysbiotic change associated with disease recurrence in the presence of sub-standard supportive periodontal therapy or other patient-related factors.
本研究的主要目的是分析治疗后侵袭性牙周炎（AgP）患者的牙周微生物组。
平均在完成积极牙周治疗后 7 年召回 66 名 AgP 患者，并采集龈下菌斑样本进行 16S rRNA 基因测序分析。
在 66 名参与者中，52 名患者表现出持续性牙周病，而 13 名患者被认为是“成功治疗的 AgP”（无探诊袋深度>4mm），1 名患者完全无牙。与持续性全口疾病相关的属包括放线菌、Alloprevotella、卡他莫拉菌、噬纤维菌、Fretibacterium、梭杆菌、螺旋体、Mogibacterium、Saccharibacteria[G-1]、唾液链球菌和密螺旋体。“成功治疗”患者中含有更高比例的嗜血杆菌、罗氏菌和罗氏菌和棒状杆菌、链球菌和肽菌。总体而言，与稳定患者相比，持续性全口 AgP（GAgP）患者的 alpha 多样性更高（p<0.001）。β多样性分析仅显示稳定和持续性 GAgP 组之间存在显著差异（p=0.004）。
与成功治疗的患者相比，持续性 AgP 患者的龈下生物膜显示出更明显的生态失调。尚需确定这些差异是否是疾病活动的易感性因素，还是与疾病复发相关的生态失调变化的结果，这种变化与亚标准支持性牙周治疗或其他与患者相关的因素有关。
