Miyakawa Kei, Ota Makoto, Sano Kaori, Momose Fumitaka, Okura Takashi, Kishida Noriko, Arita Tomoko, Suzuki Yasushi, Shirakura Masayuki, Asanuma Hideki, Watanabe Shinji, Ryo Akihide, Hasegawa Hideki
Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan.
AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
Influenza Other Respir Viruses. 2024 Dec;18(12):e70048. doi: 10.1111/irv.70048.
Rapid and safe neutralization assays are required for highly pathogenic avian influenza viruses, including a clade 2.3.4.4b H5N1 subtype recently found in cows. Here, we report a neutralization assay using luminescent virus-like particles. This assay has lower biosafety requirements and provides a larger dynamic range than conventional methods. We applied this technique to evaluate the cross-reactivity of neutralizing antibodies induced by clade 2.3.4.4b candidate vaccine viruses (CVVs) with the cow-derived H5N1 virus. Our findings indicate that these CVVs share antigenic characteristics with the cow-derived H5N1 virus, suggesting the potential efficacy of vaccines developed using these CVVs.
对于高致病性禽流感病毒，包括最近在牛身上发现的2.3.4.4b分支H5N1亚型，需要快速且安全的中和试验。在此，我们报告一种使用发光病毒样颗粒的中和试验。该试验的生物安全要求较低，并且比传统方法提供更大的动态范围。我们应用这项技术来评估由2.3.4.4b分支候选疫苗病毒（CVV）诱导的中和抗体与源自牛的H5N1病毒的交叉反应性。我们的研究结果表明，这些CVV与源自牛的H5N1病毒具有共同的抗原特征，这表明使用这些CVV开发的疫苗具有潜在效力。
