Pustylnyak Vladimir O, Perevalova Alina M, Gulyaeva Lyudmila F
Federal Research Center of Fundamental and Translational Medicine, Laboratory of molecular mechanisms of carcinogenesis, Novosibirsk, Russia.
Novosibirsk State University, Zelman Institute for the Medicine and Psychology, Novosibirsk, Russia.
Bioinform Biol Insights. 2024 Nov 28;18:11779322241302168. doi: 10.1177/11779322241302168. eCollection 2024.
MicroRNAs play a significant role in the development of cancers, including lung cancer. A recent study revealed that smoking, a key risk factor for lung cancer, increased the levels of hsa-mir-301a in the tumor tissues of patients with lung squamous cell carcinoma (LUSC). The aim of the current study is to investigate the mechanism by which tobacco smoke increases hsa-mir-301a levels in LUSC tumor tissues using bioinformatics analysis. Bioinformatics tools and online databases, including The Cancer Genome Atlas (TCGA), LinkedOmics, and Encyclopedia of RNA Interactomes (ENCORI), were applied in this study. Our results showed a correlation between the upregulation of hsa-mir-301a in LUSC tissues and smoking exposure. However, no correlation was discovered between patients' smoking status and the expression level of the hsa-mir-301a host gene, , prompting us to investigate possible changes in microRNA processing under tobacco smoke exposure. In silico results using online platforms suggest that post-transcriptional processes, which involve the RNA-binding proteins DGCR8 and FUS, contribute to the elevation of mature hsa-mir-301a levels in smoking patients with LUSC. Our findings suggest that RNA-binding proteins play a key role in controlling the processing of hsa-mir-301a, indicating a complex regulation of hsa-mir-301a in the LUSC tissues of smokers.
微小RNA在包括肺癌在内的多种癌症发展过程中发挥着重要作用。最近一项研究显示，吸烟作为肺癌的一个关键风险因素，会使肺鳞状细胞癌（LUSC）患者肿瘤组织中的hsa - mir - 301a水平升高。本研究旨在通过生物信息学分析，探究烟草烟雾增加LUSC肿瘤组织中hsa - mir - 301a水平的机制。本研究应用了多种生物信息学工具和在线数据库，包括癌症基因组图谱（TCGA）、LinkedOmics以及RNA相互作用组百科全书（ENCORI）。我们的研究结果表明，LUSC组织中hsa - mir - 301a的上调与吸烟暴露之间存在关联。然而，未发现患者的吸烟状态与hsa - mir - 301a宿主基因的表达水平之间存在相关性，这促使我们去研究烟草烟雾暴露下微小RNA加工过程中可能发生的变化。利用在线平台的计算机模拟结果表明，涉及RNA结合蛋白DGCR8和FUS的转录后过程，促成了吸烟的LUSC患者体内成熟hsa - mir - 301a水平的升高。我们的研究结果表明，RNA结合蛋白在控制hsa - mir - 301a的加工过程中起关键作用，这表明在吸烟者的LUSC组织中，hsa - mir - 301a受到复杂的调控。
