Misra Sankalp, Prasad Priti, Semwal Pradeep, Mishra Shashank Kumar, Asif Mehar Hasan, Chauhan Puneet Singh
Microbial Technologies Division, Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001 India.
Faculty of Biosciences, Institute of Biosciences and Technology, Shri Ramswaroop Memorial University, Lucknow- Deva Road, Barabanki, Uttar Pradesh 225003 India.
3 Biotech. 2024 Dec;14(12):316. doi: 10.1007/s13205-024-04164-7. Epub 2024 Nov 28.
Previous research leads have affirmed the crucial role of plant growth-promoting rhizobacteria (PGPR) strains (NBRI 12 M), (NBRI 28B, NBRI 33N), and (NBRI 13E) in salt stress amelioration and plant growth promotion. In the present study, whole-genome analysis unveiled the underlying molecular mechanisms accounting for phyto-beneficial and stress-mitigating traits of the selected PGPR strains. The genomic characterization has revealed that NBRI 12 M, NBRI 28B, NBRI 33N, and NBRI 13E possessed a single circular chromosome of 3.73 Mb, 4.07 Mb, 4.10 Mb, and 2.17 Mb size, respectively. The genome analysis of these strains demonstrated varied genes such as mrp and yfiY for plant growth promotion, nutrient metabolism, and other secondary metabolites biosynthesis. High salinity tolerance genes (yicL, ydhP_1, spoIIQ, and spoIIID), encoding for membrane transporter, dormancy, and sporulation, were also identified. In addition, several chemotaxis (cheA, cheY, and cheW) and motility gene clusters (motB_1, motB_2) were found in the PGPR strains for successful rhizosphere colonization. Further, NBRI 12 M has significantly increased the shoot and root length and dry weight by 14.13%, 20.63%, and 9.63%, respectively, under salt stress. In addition, NBRI 12 M inoculation reduced defense enzymes by 79.77%, 84.75%, 74.11%, 70.77%, and 57.75% for SOD, APX, GPX, CAT, and PPO, respectively. Overall findings from this study offered a detailed comparative genomic analysis of salt stress ameliorating PGPR of genera towards enhancing the deep insights for host-PGPR association.
The online version contains supplementary material available at 10.1007/s13205-024-04164-7.
先前的研究表明，植物促生根际细菌（PGPR）菌株（NBRI 12 M）、（NBRI 28B、NBRI 33N）和（NBRI 13E）在缓解盐胁迫和促进植物生长方面起着关键作用。在本研究中，全基因组分析揭示了所选PGPR菌株具有植物有益和减轻胁迫特性的潜在分子机制。基因组特征表明，NBRI 12 M、NBRI 28B、NBRI 33N和NBRI 13E分别拥有一条大小为3.73 Mb、4.07 Mb、4.10 Mb和2.17 Mb的单环染色体。对这些菌株的基因组分析表明，它们具有多种基因，如促进植物生长、营养代谢和其他次生代谢物生物合成的mrp和yfiY基因。还鉴定出了高耐盐性基因（yicL、ydhP_1、spoIIQ和spoIIID），这些基因编码膜转运蛋白、休眠和孢子形成相关蛋白。此外，在PGPR菌株中发现了几个趋化性（cheA、cheY和cheW）和运动基因簇（motB_1、motB_2），以实现根际的成功定殖。此外，在盐胁迫下，NBRI 12 M使地上部和根部长度以及干重分别显著增加了14.13%、20.63%和9.63%。此外，接种NBRI 12 M使超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）、谷胱甘肽过氧化物酶（GPX）、过氧化氢酶（CAT）和多酚氧化酶（PPO）的防御酶分别降低了79.77%、84.75%、74.11%、70.77%和57.75%。本研究的总体结果提供了对缓解盐胁迫的PGPR属的详细比较基因组分析，以加深对宿主与PGPR关联的深入了解。
在线版本包含可在10.1007/s13205-024-04164-7获取的补充材料。
