Nordgaard Mathilde, Blake Christopher, Maróti Gergely, Hu Guohai, Wang Yue, Strube Mikael Lenz, Kovács Ákos T
Bacterial Interactions and Evolution Group, DTU Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
Institute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network (ELKH), 6726 Szeged, Hungary.
iScience. 2022 May 14;25(6):104406. doi: 10.1016/j.isci.2022.104406. eCollection 2022 Jun 17.
is known to promote plant growth and protect plants against disease. rapidly adapts to root colonization, as evidenced by improved root colonizers already after 12 consecutive transfers between seedlings in a hydroponic setup. Re-sequencing of single evolved isolates and endpoint populations revealed mutations in genes related to different bacterial traits, in accordance with evolved isolates displaying increased root colonization associated with robust biofilm formation in response to the plant polysaccharide xylan and impaired motility. Interestingly, evolved isolates suffered a fitness disadvantage in a non-selective environment, demonstrating an evolutionary cost of adaptation to the plant root. Finally, increased root colonization by an evolved isolate was also demonstrated in the presence of resident soil microbes. Our findings highlight how a plant growth-promoting rhizobacterium rapidly adapts to an ecologically relevant environment and reveal evolutionary consequences that are fundamental to consider when evolving strains for biocontrol purposes.
已知其能促进植物生长并保护植物免受病害。它能迅速适应根部定殖，在水培装置中连续12次在幼苗间转移后，根部定殖者的改善就证明了这一点。对单个进化分离株和终点群体的重测序揭示了与不同细菌性状相关的基因中的突变，这与进化分离株显示出的因对植物多糖木聚糖有反应而增加根部定殖以及与强大生物膜形成相关且运动性受损相一致。有趣的是，进化分离株在非选择性环境中存在适应性劣势，这表明适应植物根部存在进化成本。最后，在有常驻土壤微生物存在的情况下，也证明了进化分离株增加了根部定殖。我们的研究结果突出了一种促进植物生长的根际细菌如何迅速适应生态相关环境，并揭示了在为生物防治目的进化菌株时需要考虑的基本进化后果。
