Zhang Lu, Yao Xue, Peng Min, Ma Yu, Peng Yu, Wang Ya-Wen
School of Chemistry & School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
Org Biomol Chem. 2025 Jan 15;23(3):721-727. doi: 10.1039/d4ob01599a.
A new fluorescent probe (SWJT-32) with a large Stokes shift was designed and synthesized with 2-(2-hydroxyphenyl)-4(1)-quinazolinone (HPQ) as the skeleton and ,-dimethylthiocarbamate as the recognition site. It can detect hypochlorite ions (ClO) through fluorescence spectroscopy based on the excited-state intramolecular proton transfer (ESIPT) and intramolecular charge transfer (ICT) mechanism. The probe has the advantages of strong diffusion resistance, good photostability and selectivity, and high imaging contrast, and can be used for the detection of ClO. Meanwhile, due to its low toxicity and good biocompatibility in living cells, SWJT-32 can be used for the detection of endogenous and exogenous ClO in HeLa cells as well as for long-term imaging in tumor mice.
以2-(2-羟基苯基)-4(1)-喹唑啉酮（HPQ）为骨架、二甲基硫代氨基甲酸酯为识别位点，设计合成了一种具有大斯托克斯位移的新型荧光探针（SWJT-32）。它可基于激发态分子内质子转移（ESIPT）和分子内电荷转移（ICT）机制，通过荧光光谱法检测次氯酸根离子（ClO）。该探针具有抗扩散能力强、光稳定性和选择性好、成像对比度高的优点，可用于ClO的检测。同时，由于其在活细胞中具有低毒性和良好的生物相容性，SWJT-32可用于检测HeLa细胞内源性和外源性ClO，以及用于肿瘤小鼠的长期成像。
