Hayden D W, Ghobrial H K, Johnson K H, Buoen L C
Vet Pathol. 1986 Mar;23(2):118-24. doi: 10.1177/030098588602300203.
A recurrent mammary sarcoma from an 11-year-old, female domestic cat was studied by light and electron microscopy and immunohistochemistry. The tumor consisted of interlacing bands of spindle cells with elongated blunt-ended nuclei and variable amounts of stroma. Multinucleated tumor giant cells and mitoses were common. Ultrastructurally, tumor cells had abundant rough endoplasmic reticulum with dilated cisternae, a prominent Golgi complex, frequent mitochondria, bundles of intracytoplasmic filaments with focal densities, and discontinuous basal lamina-like material and cell junctions. These findings suggest that myofibroblast-like cells were the predominant type of tumor cell. Failure to demonstrate immunoreactivity for cytokeratins attested to the non-epithelial origin of these neoplastic cells. Uniform immunoreactivity with anti-vimentin antibodies and specific labelling of some tumor cells with antiserum to actin are compatible with an origin of this tumor from modified fibroblasts (i.e., myofibroblast-like cells). Tumors composed largely of myofibroblasts may be unique and warrant separate classification from other types of fibroblastic tumors in animals.
对一只11岁雌性家猫的复发性乳腺肉瘤进行了光镜、电镜和免疫组织化学研究。肿瘤由交织的梭形细胞带组成，细胞核呈细长钝端状，间质数量不等。多核肿瘤巨细胞和有丝分裂常见。超微结构上，肿瘤细胞有丰富的粗面内质网，其池扩张，高尔基体突出，线粒体常见，有束状的胞质内细丝，有局灶性致密物，还有不连续的基底膜样物质和细胞连接。这些发现表明肌成纤维细胞样细胞是肿瘤细胞的主要类型。细胞角蛋白免疫反应性缺失证明这些肿瘤细胞非上皮起源。抗波形蛋白抗体的一致免疫反应性以及用抗肌动蛋白抗血清对一些肿瘤细胞的特异性标记与该肿瘤起源于改良的成纤维细胞（即肌成纤维细胞样细胞）相符。主要由肌成纤维细胞组成的肿瘤可能很独特，值得与动物的其他类型纤维母细胞瘤分开分类。
