de Laleijne-Liefting Lara A E, Bontekoe Ido J, Lagerberg Johan W, Klei Thomas R L
Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, the Netherlands.
Vox Sang. 2025 Mar;120(3):277-283. doi: 10.1111/vox.13778. Epub 2024 Dec 2.
Plasma components are visually inspected, and non-transparent, turbid units are rejected for transfusion and fractionation. Additionally, in case a plasma component is deemed lipaemic, there is conflicting data on the accompanying red cell concentrate (RCC) in vitro quality. As visual inspection of plasma turbidity is a subjective method, we aimed to devise an objective measurement using a quick, non-invasive, table-top spectrophotometry-based method. Using this method, the correlation between spectrophotometric data and its predictive value on haemolysis of the accompanying RCC during storage was assessed.
A total of 365 plasma units were visually inspected for turbidity and analysed for light reflection parameters (L*, a* and b*) and triglyceride (TG) levels. Leukoreduced RCCs in saline-adenine-glucose-mannitol (SAGM), prepared from the accompanying lipaemic whole blood, were stored for up to 6 weeks and analysed for quality parameters.
The light reflection L* value was the most discriminating between clear and turbid/lipaemic plasma. Also, a correlation was found between TG levels and L* values (R = 0.703). Plasma with TG ≥ 2.5 mmol/L showed an L* value >50 with >90% specificity and sensitivity. RCC from donations with a plasma L* value ≥68 showed significantly higher haemolysis levels (p < 0.05) during storage.
The non-invasive photometric analysis of plasma turbidity correlated both with visual inspection and plasma TG levels. Measurement of L* values of plasma may be helpful in identifying donations with high TG levels and higher risk for increased haemolysis during RCC storage.
对血浆成分进行目视检查，不透明、浑浊的单位被拒绝用于输血和分馏。此外，对于脂血的血浆成分，其伴随的红细胞浓缩液（RCC）体外质量的数据存在矛盾。由于血浆浑浊度的目视检查是一种主观方法，我们旨在设计一种基于快速、非侵入性台式分光光度法的客观测量方法。使用该方法，评估分光光度数据与其对储存期间伴随RCC溶血的预测价值之间的相关性。
共对365个血浆单位进行浑浊度目视检查，并分析其光反射参数（L*、a和b）以及甘油三酯（TG）水平。从伴随的脂血全血制备的白细胞滤除的RCC在生理盐水 - 腺嘌呤 - 葡萄糖 - 甘露醇（SAGM）中储存长达6周，并分析质量参数。
光反射L值在清澈与浑浊/脂血血浆之间的区分度最高。此外，发现TG水平与L值之间存在相关性（R = 0.703）。TG≥2.5 mmol/L的血浆L值>50，特异性和敏感性>90%。血浆L值≥68的献血者的RCC在储存期间溶血水平显著更高（p < 0.05）。
血浆浑浊度的非侵入性光度分析与目视检查和血浆TG水平均相关。测量血浆的L*值可能有助于识别TG水平高且RCC储存期间溶血增加风险更高的献血。
