Al-Abdullatif Abdulaziz A, Alhotan Rashed A, Al-Badwi Mohammed A, Dong Xinyang, Kettunen Hannele, Vuorenmaa Juhani, Sakr Shimaa A, Azzam Mahmoud M
Animal Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
Animal Science College, Zhejiang University, Zijingang Campus, Hangzhou, China.
Front Vet Sci. 2024 Nov 18;11:1484150. doi: 10.3389/fvets.2024.1484150. eCollection 2024.
The objective of this study was to enhance the knowledge about the effects of hydrolyzed yeast supplementation on growth performance, woody breast myopathy, and its mechanism on intestinal homeostasis using antioxidant and immunomodulatory-related gene expressions in heat-stressed broiler chickens. In a 35-d feeding experiment, 160-day-old male Ross 308 broiler chickens were assigned to four dietary groups, consisting of eight replicates and five birds per replicate. Experimental diets contained four levels of hydrolyzed yeast (HY) (0, 400, 800, or 1,200 mg.kg) derived from . On d 25, birds were exposed to cyclic heat stress (HS) (35°C for 8 h/d from 8 a.m. to 4.00 p.m.) for 10 days. Adding HY at 800 mg.kg numerically decreased the feed conversion ratio (FCR) on days 25-35 (heat stress period) by 2.50%. Furthermore, the addition of HY reduced ( = 0.005) mortality rate compared with those of birds fed the control diet. Supplementation of HY exhibited efficacy ( = 0.09) in diminishing woody breast (WB) in terms of incidence and degree of severity. Furthermore, the added HY decreased ( < 0.001) drip loss values of the compared with the control diet group; the addition of HY at 400 and 1,200 mg.kg decreased ( < 0.001) cooking loss values in the . In addition, HY supplementation at 800 mg.kg decreased ( = 0.04) the duodenal mRNA expression of the avian β-defensin 10 (AvBD10) and increased ( < 0.05) the mRNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and secreted immunoglobulin A (sIgA). The addition of HY at 400 and 800 mg.kg decreased ( = 0.001) the duodenal mRNA expression of copper and zinc superoxide dismutase (Cu-ZnSOD1). HY supplementation tended to decrease ( =0.07) the duodenal mRNA expression of heat shock protein 70 (HSP70). The results suggest that hydrolyzed yeast supplementation to broiler chickens exposed to heat stress might improve intestinal redox homeostasis and decrease the mortality rate. The inclusion of 800 mg.kg HY in the diet enhanced duodenal redox homeostasis, while 400-1,200 mg.kg HY reduced mortality rate and exhibited lower drip loss values and reduced woody breast of in terms of incidence and degree of severity.
本研究的目的是通过热应激肉鸡中抗氧化和免疫调节相关基因的表达，增强对水解酵母补充剂对生长性能、木胸肌病及其对肠道稳态机制影响的认识。在一项为期35天的饲养实验中，将160日龄的雄性罗斯308肉鸡分为四个日粮组，每组8个重复，每个重复5只鸡。实验日粮含有四种水平的水解酵母（HY）（0、400、800或1200毫克/千克），来源为……。在第25天，鸡只暴露于循环热应激（HS）（从上午8点到下午4点，35°C，每天8小时）10天。在第25至35天（热应激期）添加800毫克/千克的HY使饲料转化率（FCR）在数值上降低了2.50%。此外，与饲喂对照日粮的鸡相比，添加HY降低了（P = 0.005）死亡率。补充HY在降低木胸（WB）的发病率和严重程度方面显示出效果（P = 0.09）。此外，与对照日粮组相比，添加HY降低了（P < 0.001）胸肉的滴水损失值；添加400和1200毫克/千克的HY降低了（P < 0.001）胸肉的蒸煮损失值。此外，添加800毫克/千克的HY降低了（P = 0.04）禽β-防御素10（AvBD10）的十二指肠mRNA表达，并增加了（P < 0.05）核因子红细胞2相关因子2（Nrf2）、活化B细胞核因子κ轻链增强子（NF-κB）和分泌型免疫球蛋白A（sIgA）的mRNA表达。添加400和800毫克/千克的HY降低了（P = 0.001）铜锌超氧化物歧化酶（Cu-ZnSOD1）的十二指肠mRNA表达。补充HY倾向于降低（P = 0.07）热休克蛋白70（HSP70）的十二指肠mRNA表达。结果表明，对热应激肉鸡补充水解酵母可能改善肠道氧化还原稳态并降低死亡率。日粮中添加800毫克/千克的HY增强了十二指肠氧化还原稳态，而400至1200毫克/千克的HY降低了死亡率，并在胸肉的滴水损失值和木胸的发病率及严重程度方面表现出较低水平。
